{"product_id":"phospho-beta-arrestin-1-ser412-antibody-arrb1-bha17136141","title":"Phospho-Beta Arrestin 1 (Ser412) Antibody \/ ARRB1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Beta Arrestin 1 (Ser412) Antibody \/ ARRB1 is a anti-ARRB1 (phospho-Ser412) Rabbit antibody Recombinant Rabbit Monoclonal clone 32A60 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARRB1 (phospho site: Ser412)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32A60, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Beta Arrestin 1 (Ser412) antibody detects Beta arrestin 1 phosphorylated at serine 412, encoded by the ARRB1 gene. Beta arrestin 1 is a multifunctional adaptor protein best known for its role in G protein coupled receptor desensitization and internalization. Phosphorylation at serine 412 represents a key regulatory modification that influences receptor trafficking, signaling scaffolds, and cellular responses to extracellular stimuli. Phospho-Beta Arrestin 1 (Ser412) antibody provides a highly specific tool for dissecting the dynamic regulation of G protein coupled receptor signaling and arrestin mediated pathways.\u003cbr\u003e\u003cbr\u003eBeta arrestin 1 functions by binding activated, phosphorylated G protein coupled receptors, preventing further coupling to heterotrimeric G proteins while simultaneously recruiting clathrin and adaptor proteins to promote endocytosis. Research using Phospho-Beta Arrestin 1 (Ser412) antibody has demonstrated that phosphorylation at serine 412 modulates the strength and duration of this interaction, acting as a molecular switch between receptor desensitization and alternative signaling functions. This modification influences the balance between canonical and non canonical pathways initiated at the plasma membrane.\u003cbr\u003e\u003cbr\u003eBeyond receptor internalization, Beta arrestin 1 acts as a scaffold for signaling cascades including MAPK, AKT, and NF kappa B pathways. Studies with Phospho-Beta Arrestin 1 (Ser412) antibody have shown that phosphorylation at serine 412 alters scaffold assembly, thereby regulating downstream signaling specificity. This makes it an essential modification for determining cellular outcomes in response to hormones, neurotransmitters, and growth factors.\u003cbr\u003e\u003cbr\u003eDysregulation of Beta arrestin 1 phosphorylation has been linked to disease. Research using Phospho-Beta Arrestin 1 (Ser412) antibody has revealed that altered phosphorylation patterns contribute to cardiovascular disease, neurological disorders, and cancer. In heart failure, aberrant phosphorylation disrupts receptor regulation, while in cancer, changes in Beta arrestin 1 signaling affect cell proliferation and metastasis. These observations underscore the importance of precise phosphorylation control in health and pathology.\u003cbr\u003e\u003cbr\u003ePhospho-Beta Arrestin 1 (Ser412) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms phosphorylation status, immunohistochemistry highlights tissue specific activation patterns, and immunofluorescence demonstrates subcellular localization of phosphorylated arrestin in endosomes and receptor complexes. These experimental approaches make the antibody indispensable for signal transduction research.\u003cbr\u003e\u003cbr\u003eBy providing validated Phospho-Beta Arrestin 1 (Ser412) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARRB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARRB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARRB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARRB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARRB1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=ARRB1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047319363949,"sku":"FY13239","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7f3dcb98-e3c9-445d-bd0b-563afd90438a.jpg?v=1772019415","url":"https:\/\/www.ebiohippo.com\/products\/phospho-beta-arrestin-1-ser412-antibody-arrb1-bha17136141","provider":"BioHippo","version":"1.0","type":"link"}