{"product_id":"phospho-beta-catenin-thr41-ser45-antibody-bha17135402","title":"Phospho-Beta Catenin (Thr41\/Ser45) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Beta Catenin (Thr41\/Ser45) Antibody is a anti-Beta Catenin (phospho-Thr41, Ser45) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C69 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Beta Catenin (phospho site: Thr41, Ser45)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C69, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Beta Catenin (Thr41\/Ser45) antibody detects beta catenin phosphorylated at threonine 41 and serine 45. Beta catenin, encoded by the CTNNB1 gene, is a multifunctional protein involved in cell adhesion and Wnt signaling. In adherens junctions, beta catenin binds to cadherins and links them to the actin cytoskeleton. In the Wnt pathway, beta catenin functions as a transcriptional coactivator that regulates genes controlling proliferation and differentiation. Phosphorylation at Thr41 and Ser45 targets beta catenin for ubiquitination and proteasomal degradation, providing a key mechanism for pathway regulation.\u003cbr\u003e\u003cbr\u003ePhospho-Beta Catenin (Thr41\/Ser45) antibody is widely used in cancer research, developmental biology, and signaling studies. Aberrant stabilization of beta catenin due to impaired phosphorylation leads to constitutive activation of Wnt signaling and is a hallmark of many cancers. By detecting phosphorylated beta catenin, this antibody enables monitoring of degradation signals and pathway regulation.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. Western blot assays with Phospho-Beta Catenin (Thr41\/Ser45) antibody reveal phosphorylated bands distinct from non phosphorylated forms. Immunohistochemistry maps phospho beta catenin in tissues, while immunofluorescence highlights subcellular localization at membranes and cytoplasm under conditions of Wnt regulation. These applications provide high resolution insight into signaling events.\u003cbr\u003e\u003cbr\u003ePhosphorylation of beta catenin at Thr41 and Ser45 is mediated by casein kinase I and glycogen synthase kinase 3, initiating a cascade of phosphorylation events that promote its degradation. Dysregulation of this process contributes to tumorigenesis, particularly in colorectal, liver, and ovarian cancers. By using Phospho-Beta Catenin (Thr41\/Ser45) antibody, researchers can evaluate how phosphorylation dependent regulation of CTNNB1 impacts oncogenic signaling.\u003cbr\u003e\u003cbr\u003eBeyond oncology, Wnt beta catenin signaling plays roles in stem cell biology, embryogenesis, and tissue regeneration. Monitoring phosphorylation at key sites provides mechanistic understanding of pathway control in diverse contexts. The phospho specific antibody therefore serves as a critical reagent for investigating developmental biology and regenerative medicine.\u003cbr\u003e\u003cbr\u003ePhospho-Beta Catenin (Thr41\/Ser45) antibody provided by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Beta+Catenin - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Beta+Catenin - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Beta+Catenin - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Beta+Catenin - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Beta+Catenin+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=Beta+Catenin --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047291347309,"sku":"FY12499","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_40b1beab-1707-4378-ad19-62b417944491.jpg?v=1772019300","url":"https:\/\/www.ebiohippo.com\/products\/phospho-beta-catenin-thr41-ser45-antibody-bha17135402","provider":"BioHippo","version":"1.0","type":"link"}