{"product_id":"phospho-c-jun-jund-ser73-ser100-antibody-bha17135471","title":"Phospho-c-Jun\/JunD (Ser73\/Ser100) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-c-Jun\/JunD (Ser73\/Ser100) Antibody is a anti-c-Jun\/JunD (phospho-Ser73, Ser100) Rabbit antibody Recombinant Rabbit Monoclonal clone 30J61 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e c-Jun\/JunD (phospho site: Ser73, Ser100)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30J61, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-c-Jun\/JunD (Ser73\/Ser100) antibody detects the phosphorylated forms of c-Jun and JunD, transcription factors belonging to the activator protein 1 (AP-1) family. c-Jun, encoded by the JUN gene, and JunD, encoded by the JUND gene, regulate gene expression in response to growth factors, stress signals, and cytokines. Phosphorylation at serine 73 in c-Jun and serine 100 in JunD is mediated by JNK kinases, enhancing transcriptional activity by promoting interaction with co-activators and stabilization of AP-1 complexes. These phosphorylation events are critical for cell proliferation, apoptosis, and stress responses.\u003cbr\u003e\u003cbr\u003ePhospho-c-Jun\/JunD (Ser73\/Ser100) antibody is widely applied in cancer biology, immunology, and stress signaling research. Detection of phosphorylated AP-1 proteins provides insight into MAPK signaling cascades that drive transcriptional reprogramming. Abnormal AP-1 activation contributes to oncogenesis, inflammation, and neurodegeneration. By detecting phosphorylated c-Jun and JunD, researchers can examine how stress-activated kinases influence gene regulation and disease progression.\u003cbr\u003e\u003cbr\u003e Western blot assays reveal phosphorylated isoforms distinct from non-phosphorylated proteins. Immunohistochemistry maps AP-1 activation across tissues, while immunofluorescence highlights nuclear localization of phosphorylated c-Jun and JunD. These techniques allow high-resolution tracking of transcription factor activation in diverse biological contexts.\u003cbr\u003e\u003cbr\u003ePhosphorylation of c-Jun and JunD integrates signals from growth factors, UV radiation, oxidative stress, and cytokines. Sustained phosphorylation is often linked to tumorigenesis and resistance to therapy, while transient activation supports adaptive responses. By applying Phospho-c-Jun\/JunD (Ser73\/Ser100) antibody, scientists can evaluate pathway dynamics and investigate therapeutic strategies targeting JNK-AP1 signaling in cancer and inflammation.\u003cbr\u003e\u003cbr\u003eBeyond oncology, phosphorylated AP-1 proteins play roles in embryonic development, neuronal plasticity, and immune activation. Their regulation of genes involved in apoptosis, differentiation, and repair underscores their biological importance. The phospho-specific antibody therefore provides a versatile reagent for dissecting cellular responses to external and internal cues.\u003cbr\u003e\u003cbr\u003ePhospho-c-Jun\/JunD (Ser73\/Ser100) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=c-Jun\/JunD - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=c-Jun\/JunD - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=c-Jun\/JunD - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/c-Jun\/JunD - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=c-Jun\/JunD+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=c-Jun\/JunD --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047293247853,"sku":"FY12568","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_19cf61cb-fc49-4e47-94ca-fcce173dc65d.jpg?v=1772019308","url":"https:\/\/www.ebiohippo.com\/products\/phospho-c-jun-jund-ser73-ser100-antibody-bha17135471","provider":"BioHippo","version":"1.0","type":"link"}