{"product_id":"phospho-camkii-alpha-t286-antibody-camk2a-bha17135730","title":"Phospho-CaMKII alpha (T286) Antibody \/ CAMK2A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-CaMKII alpha (T286) Antibody \/ CAMK2A is a anti-CAMK2A (phospho-Thr286) Rabbit antibody Recombinant Rabbit Monoclonal clone 31C26 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CAMK2A (phospho site: Thr286)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31C26, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-CaMKII alpha (T286) antibody detects calcium\/calmodulin-dependent protein kinase II alpha when phosphorylated at threonine 286. The protein is encoded by the CAMK2A gene and is commonly referred to as CaM kinase II alpha, CaMK-II subunit alpha, and calcium\/calmodulin-dependent protein kinase type II alpha chain. CaMKII alpha is a serine\/threonine kinase expressed abundantly in the brain, where it regulates synaptic plasticity, learning, and memory. Autophosphorylation at threonine 286 converts the kinase into a calcium\/calmodulin-independent active form, a hallmark event in long-term potentiation and neuronal signaling.\u003cbr\u003e\u003cbr\u003ePhospho-CaMKII alpha (T286) antibody is widely applied in neuroscience, synaptic biology, and signaling research. Detecting phosphorylation at this site provides a direct measure of CaMKII activation, linking cellular activity to memory formation. In neurons, Thr286 phosphorylation prolongs kinase activity beyond transient calcium spikes, enabling sustained synaptic strengthening. By targeting this phosphorylation event, researchers can explore how activity-dependent signaling translates into lasting changes in connectivity.\u003cbr\u003e\u003cbr\u003eApplications for Phospho-CaMKII alpha (T286) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated CaMKII alpha isoforms in brain lysates, immunohistochemistry maps activation in hippocampus and cortex, and immunofluorescence reveals localization at dendritic spines. These methods provide detailed views of synaptic signaling in vitro and in vivo.\u003cbr\u003e\u003cbr\u003ePhosphorylation at Thr286 has been extensively studied in relation to long-term potentiation, a cellular mechanism of memory storage. Abnormal regulation of CaMKII phosphorylation contributes to neurological disorders, including Alzheimer disease, schizophrenia, and epilepsy. By applying Phospho-CaMKII alpha (T286) antibody, scientists can investigate disease-related disruptions in synaptic plasticity.\u003cbr\u003e\u003cbr\u003eBeyond neuroscience, CaMKII alpha also participates in cardiac signaling and metabolism. Phosphorylation at Thr286 has been detected in cardiac stress responses and metabolic regulation. The antibody therefore supports research into CaMKII biology across systems.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CAMK2A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CAMK2A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CAMK2A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CAMK2A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CAMK2A+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=CAMK2A --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302750573,"sku":"FY12828","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_87743db4-301a-41d8-b76d-bc44abca337d.jpg?v=1772019351","url":"https:\/\/www.ebiohippo.com\/products\/phospho-camkii-alpha-t286-antibody-camk2a-bha17135730","provider":"BioHippo","version":"1.0","type":"link"}