{"product_id":"phospho-chk1-ser280-antibody-checkpoint-kinase-1-bha17135399","title":"Phospho-Chk1 (Ser280) Antibody \/ Checkpoint kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Chk1 (Ser280) Antibody \/ Checkpoint kinase 1 is a anti-CHK1 (phospho-Ser280) Rabbit antibody Recombinant Rabbit Monoclonal clone 31C46 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CHK1 (phospho site: Ser280)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31C46, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Chk1 (Ser280) antibody detects checkpoint kinase 1 when phosphorylated at serine 280. Chk1 is a serine threonine kinase encoded by the CHEK1 gene and functions as a key regulator of the DNA damage response. Phosphorylation at Ser280 influences subcellular localization and activity, thereby modulating cell cycle progression and checkpoint control. This modification integrates signals from replication stress and genotoxic damage, ensuring genomic stability.\u003cbr\u003e\u003cbr\u003ePhospho-Chk1 (Ser280) antibody is widely used in DNA damage, cell cycle, and cancer research. By specifically detecting the phosphorylated form, it enables distinction between active checkpoint signaling and basal kinase levels. Chk1 activation at Ser280 supports ATR mediated responses to stalled replication forks, allowing cells to pause progression and repair damage.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. Western blot assays reveal bands corresponding to phosphorylated Chk1 distinct from unmodified forms. Immunohistochemistry maps phosphorylation patterns within tissues, while immunofluorescence highlights nuclear distribution during checkpoint activation. These tools allow precise monitoring of checkpoint signaling in experimental systems.\u003cbr\u003e\u003cbr\u003eDysregulation of Chk1 phosphorylation contributes to oncogenesis by enabling survival of cells with damaged DNA. Elevated phospho Chk1 levels have been observed in tumors, where they correlate with therapy resistance. By applying Phospho-Chk1 (Ser280) antibody, scientists can explore how checkpoint signaling influences cancer progression and therapeutic outcomes. It is also valuable for evaluating Chk1 inhibitors under development as cancer treatments.\u003cbr\u003e\u003cbr\u003eBeyond cancer, Chk1 phosphorylation at Ser280 plays roles in embryonic development and stem cell biology, where it maintains genome stability during rapid proliferation. The phospho specific antibody provides a useful tool for probing these functions in developmental and regenerative contexts.\u003cbr\u003e\u003cbr\u003ePhospho-Chk1 (Ser280) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CHK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CHK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CHK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CHK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CHK1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=CHK1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047289643373,"sku":"FY12496","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_376dbc58-d103-4341-8551-35c7124ed1c1.jpg?v=1772019299","url":"https:\/\/www.ebiohippo.com\/products\/phospho-chk1-ser280-antibody-checkpoint-kinase-1-bha17135399","provider":"BioHippo","version":"1.0","type":"link"}