{"product_id":"phospho-crkl-tyr207-antibody-crk-like-protein-bha17135850","title":"Phospho-CrkL (Tyr207) Antibody \/ Crk like protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-CrkL (Tyr207) Antibody \/ Crk like protein is a anti-CrkL (phospho-Tyr207) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C08 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CrkL (phospho site: Tyr207)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C08, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-CrkL (Tyr207) antibody detects the phosphorylated form of Crk like protein, encoded by the CRKL gene. Crk like is an adaptor protein that mediates intracellular signaling downstream of multiple tyrosine kinases, including BCR ABL, EGFR, and PDGFR. It contains SH2 and SH3 domains that allow it to bind phosphotyrosine residues and recruit effector proteins, thereby linking receptor activation to downstream pathways. Phosphorylation at tyrosine 207 is a regulatory modification that influences Crk like protein interactions and signaling capacity. Phospho-CrkL (Tyr207) antibody provides researchers with a reliable reagent to study tyrosine kinase signaling in normal and malignant cells.\u003cbr\u003e\u003cbr\u003eCrk like phosphorylation at tyrosine 207 is mediated by kinases such as ABL and has been shown to modulate protein conformation and adaptor activity. Research using Phospho-CrkL (Tyr207) antibody has demonstrated that phosphorylation reduces binding to certain partners while promoting interactions with negative regulators, thus acting as a feedback mechanism to control signaling intensity. This fine tuning ensures that pathways such as Ras, Rac, and PI3K are activated appropriately in response to growth factor stimulation or stress signals.\u003cbr\u003e\u003cbr\u003eAberrant phosphorylation of Crk like at tyrosine 207 has been strongly associated with leukemia, particularly chronic myeloid leukemia, where BCR ABL fusion kinase constitutively phosphorylates this residue. Phospho-CrkL (Tyr207) antibody has therefore become a surrogate marker for BCR ABL activity in both research and clinical contexts. Monitoring Crk like phosphorylation provides a sensitive measure of kinase inhibition in patients treated with tyrosine kinase inhibitors, making it a valuable biomarker for therapeutic response. Beyond leukemia, altered phosphorylation has been observed in solid tumors, linking Crk like to diverse oncogenic pathways.\u003cbr\u003e\u003cbr\u003ePhospho-CrkL (Tyr207) antibody is used in western blotting, immunoprecipitation, and flow cytometry. Western blotting provides detection of phosphorylated protein as an indicator of kinase activity. Immunoprecipitation with this antibody isolates Crk like protein complexes, allowing characterization of binding partners under different phosphorylation states. Flow cytometry applications extend its use to patient samples, where phosphorylation levels can be measured at the single cell level. These diverse applications demonstrate the versatility of Phospho-CrkL (Tyr207) antibody in both laboratory research and translational medicine.\u003cbr\u003e\u003cbr\u003eBy providing Phospho-CrkL (Tyr207) antibody,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CrkL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CrkL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CrkL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CrkL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CrkL+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=CrkL --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047307338093,"sku":"FY12948","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ebbdc4a9-0b46-4cb2-b38c-005ac993eaf3.jpg?v=1772019368","url":"https:\/\/www.ebiohippo.com\/products\/phospho-crkl-tyr207-antibody-crk-like-protein-bha17135850","provider":"BioHippo","version":"1.0","type":"link"}