{"product_id":"phospho-eif4ebp1-thr46-antibody-eukaryotic-translation-initiation-factor-4e-binding-protein-1-bha17135323","title":"Phospho-eIF4EBP1 (Thr46) Antibody \/ Eukaryotic translation initiation factor 4E binding protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-eIF4EBP1 (Thr46) Antibody \/ Eukaryotic translation initiation factor 4E binding protein 1 is a anti-EIF4EBP1 (phospho-Thr46) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E52 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF4EBP1 (phospho site: Thr46)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32E52, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-eIF4EBP1 (Thr46) antibody detects eukaryotic translation initiation factor 4E binding protein 1 when phosphorylated at threonine 46. EIF4EBP1 is encoded by the EIF4EBP1 gene and is part of a small family of translational repressors that also includes EIF4EBP2 and EIF4EBP3. These proteins function as regulators of cap dependent translation by binding to eIF4E and preventing assembly of the translation initiation complex. Phosphorylation of EIF4EBP1 at Thr46 and other regulatory sites causes its release from eIF4E, thereby promoting translation initiation.\u003cbr\u003e\u003cbr\u003ePhospho-eIF4EBP1 (Thr46) antibody is widely applied in studies of mTOR signaling, translation control, and cancer biology. The mTOR pathway is a central regulator of cell growth, proliferation, and metabolism, and EIF4EBP1 phosphorylation is one of its key downstream events. By specifically detecting EIF4EBP1 phosphorylated at Thr46, this antibody provides a reliable marker of pathway activation and translational regulation.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-eIF4EBP1 (Thr46) antibody identifies phosphorylated protein bands distinct from non phosphorylated forms, allowing quantification of activation states. Immunohistochemistry provides tissue level mapping of phosphorylation patterns, while immunofluorescence reveals subcellular localization of activated EIF4EBP1. These applications make the antibody valuable for monitoring mTOR activity across experimental models.\u003cbr\u003e\u003cbr\u003eEIF4EBP1 phosphorylation at Thr46 is conserved among family members, and antibodies raised against this site may also detect EIF4EBP2 at Thr46 and EIF4EBP3 at Thr32. This cross reactivity can be useful in capturing broader regulation of translation initiation but should be considered in experimental design. By applying Phospho-eIF4EBP1 (Thr46) antibody, researchers can evaluate translational control across multiple EIF4EBP proteins.\u003cbr\u003e\u003cbr\u003eDysregulation of EIF4EBP1 phosphorylation is common in cancers, where hyperactivation of mTOR signaling drives uncontrolled growth and survival. Detection of phospho EIF4EBP1 is frequently used as a biomarker of therapeutic response to mTOR inhibitors. Beyond oncology, mTOR EIF4EBP1 signaling regulates metabolism, neuronal plasticity, and immune responses, expanding the relevance of this antibody to diverse research fields.\u003cbr\u003e\u003cbr\u003ePhospho-eIF4EBP1 (Thr46) antibody provided by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF4EBP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF4EBP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF4EBP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF4EBP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF4EBP1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=EIF4EBP1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047288562029,"sku":"FY12420","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c3732df9-871a-47ad-bb0d-f4e1540109c7.jpg?v=1782237020","url":"https:\/\/www.ebiohippo.com\/products\/phospho-eif4ebp1-thr46-antibody-eukaryotic-translation-initiation-factor-4e-binding-protein-1-bha17135323","provider":"BioHippo","version":"1.0","type":"link"}