{"product_id":"phospho-eif4ebp1-thr70-antibody-eif4ebp1-bha17136054","title":"Phospho-eIF4EBP1 (Thr70) Antibody \/ EIF4EBP1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-eIF4EBP1 (Thr70) Antibody \/ EIF4EBP1 is a anti-EIF4EBP1 (phospho-Thr70) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E48 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF4EBP1 (phospho site: Thr70)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32E48, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-eIF4EBP1 (Thr70) antibody detects Eukaryotic translation initiation factor 4E binding protein 1 phosphorylated at threonine 70, encoded by the EIF4EBP1 gene. This protein is a central regulator of cap-dependent translation initiation, acting as a repressor by binding eIF4E and preventing assembly of the eIF4F complex. Phosphorylation of eIF4EBP1 at Thr70 reduces its affinity for eIF4E, releasing the inhibition and promoting translation. Phospho-eIF4EBP1 (Thr70) antibody provides researchers with a selective reagent for monitoring mTOR pathway activity and protein synthesis regulation.\u003cbr\u003e\u003cbr\u003eEukaryotic translation initiation factor 4E binding protein 1 is one of several eIF4E binding proteins that control translation initiation in response to nutrient, growth factor, and stress signals. Research using Phospho-eIF4EBP1 (Thr70) antibody has demonstrated that Thr70 phosphorylation is a key regulatory step mediated by mTOR complex 1. This modification, along with phosphorylation at Thr37, Thr46, and Ser65, sequentially decreases binding to eIF4E. The cumulative effect is activation of translation initiation and enhanced protein synthesis, particularly for transcripts encoding growth-promoting and survival proteins.\u003cbr\u003e\u003cbr\u003eDysregulation of EIF4EBP1 phosphorylation has been linked to cancer, metabolic disease, and neurodegeneration. Studies with Phospho-eIF4EBP1 (Thr70) antibody have shown that hyperactivation of mTOR signaling leads to persistent phosphorylation of eIF4EBP1, contributing to uncontrolled translation and oncogenesis. In contrast, reduced phosphorylation in stress conditions suppresses translation, conserving resources. Because translation initiation is a central control point in gene expression, phospho-eIF4EBP1 serves as a valuable biomarker of cellular signaling status.\u003cbr\u003e\u003cbr\u003eIn oncology, high levels of phosphorylated eIF4EBP1 correlate with tumor progression, therapeutic resistance, and poor prognosis. Research using Phospho-eIF4EBP1 (Thr70) antibody has confirmed that cancer cells exploit mTOR-driven phosphorylation to promote protein synthesis needed for growth and survival. Inhibitors of mTOR restore eIF4EBP1 binding to eIF4E, suppressing translation and reducing tumor growth. These findings make detection of phosphorylation at Thr70 essential in both basic research and therapeutic monitoring.\u003cbr\u003e\u003cbr\u003ePhospho-eIF4EBP1 (Thr70) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies phosphorylation state-specific bands, immunohistochemistry reveals phosphorylation in tumor tissues, and immunofluorescence demonstrates dynamic responses to nutrient and drug treatments. These methods make Phospho-eIF4EBP1 (Thr70) antibody a critical reagent for investigating translational control.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-eIF4EBP1 (Thr70) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF4EBP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF4EBP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF4EBP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF4EBP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF4EBP1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=EIF4EBP1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047313858925,"sku":"FY13152","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9e0da00b-2eac-41a8-8aec-4719b7cb1f50.jpg?v=1782237098","url":"https:\/\/www.ebiohippo.com\/products\/phospho-eif4ebp1-thr70-antibody-eif4ebp1-bha17136054","provider":"BioHippo","version":"1.0","type":"link"}