{"product_id":"phospho-erbb2-tyr1221-tyr1222-antibody-her2-neu-bha17135669","title":"Phospho-ErbB2 (Tyr1221\/Tyr1222) Antibody \/ HER2 \/ NEU","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-ErbB2 (Tyr1221\/Tyr1222) Antibody \/ HER2 \/ NEU is a anti-ERBB2 (phospho-Tyr1221, Tyr1222) Rabbit antibody Recombinant Rabbit Monoclonal clone 31E43 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ERBB2 (phospho site: Tyr1221, Tyr1222)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31E43, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-ErbB2 (Tyr1221\/Tyr1222) antibody detects ErbB2, encoded by the ERBB2 gene, when phosphorylated at tyrosines 1221 and 1222. ErbB2 is also commonly called HER2, NEU, proto oncogene Neu, and CD340. It is a member of the epidermal growth factor receptor family of receptor tyrosine kinases. Unlike other family members, ErbB2 has no direct ligand but is the preferred dimerization partner for EGFR, ErbB3, and ErbB4. Upon dimerization, its intracellular tyrosine kinase domain autophosphorylates specific residues, including Tyr1221 and Tyr1222. These phosphorylation sites recruit adaptor proteins such as Grb2 and Shc, activating MAPK and PI3K pathways that drive cell proliferation, survival, and differentiation.\u003cbr\u003e\u003cbr\u003ePhospho-ErbB2 (Tyr1221\/Tyr1222) antibody is widely applied in cancer biology, molecular signaling, and translational research. HER2 amplification and overexpression are defining features of aggressive breast cancer subtypes, as well as subsets of gastric, ovarian, and lung cancers. Detecting phosphorylation at Tyr1221 and Tyr1222 provides direct evidence of receptor activation, going beyond total expression levels. This allows researchers to distinguish tumors with active HER2 signaling from those with mere overexpression.\u003cbr\u003e\u003cbr\u003eValidated applications for Phospho-ErbB2 (Tyr1221\/Tyr1222) antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, it identifies activated HER2 distinct from total receptor. Immunohistochemistry highlights receptor activation in tumor tissue sections, while immunofluorescence visualizes membrane localized receptor complexes in cultured cells. Together, these methods provide comprehensive tools for studying receptor activity at multiple biological scales.\u003cbr\u003e\u003cbr\u003ePhosphorylation of HER2 at Tyr1221 and Tyr1222 is central to downstream signaling. These residues recruit adaptors that activate the Ras MAPK cascade, promoting proliferation, and the PI3K Akt pathway, enhancing survival and metabolism. Persistent phosphorylation drives oncogenesis by conferring growth advantage, resistance to apoptosis, and metastatic potential. Phospho-ErbB2 (Tyr1221\/Tyr1222) antibody enables researchers to quantify this activation and study how it is regulated by dimerization partners, extracellular ligands, and therapeutic inhibitors.\u003cbr\u003e\u003cbr\u003eHER2 targeted therapies, including trastuzumab, pertuzumab, and tyrosine kinase inhibitors such as lapatinib, directly modulate phosphorylation at these sites. Monitoring Tyr1221 and Tyr1222 phosphorylation provides a pharmacodynamic biomarker of drug response. This application is critical in translational medicine, where phospho specific antibodies can guide therapeutic development and precision oncology strategies.\u003cbr\u003e\u003cbr\u003eHER2 signaling extends beyond cancer. It plays roles in cardiac physiology, where HER2 signaling supports cardiomyocyte survival, and in development, where it regulates tissue morphogenesis. Abnormal phosphorylation patterns may therefore impact not only tumors but also cardiac and developmental biology. Phospho-ErbB2 (Tyr1221\/Tyr1222) antibody supports research across these diverse biological areas.\u003cbr\u003e\u003cbr\u003ePhospho-ErbB2 (Tyr1221\/Tyr1222) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ERBB2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ERBB2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ERBB2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ERBB2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ERBB2+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=ERBB2 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047298752877,"sku":"FY12766","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_851cd34a-ac6f-4b85-aab0-be14609e5ae5.jpg?v=1772019339","url":"https:\/\/www.ebiohippo.com\/products\/phospho-erbb2-tyr1221-tyr1222-antibody-her2-neu-bha17135669","provider":"BioHippo","version":"1.0","type":"link"}