{"product_id":"phospho-erbb3-tyr1289-antibody-her3-bha17135215","title":"Phospho-ErbB3 (Tyr1289) Antibody \/ HER3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-ErbB3 (Tyr1289) Antibody \/ HER3 is a anti-ERBB3 (phospho-Tyr1289) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E64 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ERBB3 (phospho site: Tyr1289)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32E64, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-ErbB3 (Tyr1289) antibody is designed to detect the ErbB3 receptor tyrosine kinase when phosphorylated at tyrosine 1289. ErbB3, also known as HER3, is a member of the epidermal growth factor receptor (EGFR\/ErbB) family. Unlike other ErbB receptors, ErbB3 has impaired kinase activity and functions primarily through heterodimerization with other ErbB family members such as ErbB2\/HER2. Upon ligand binding, ErbB3 undergoes tyrosine phosphorylation at multiple sites, including Tyr1289, which serves as a key docking site for downstream signaling molecules like PI3K. This phosphorylation event is central to activation of the PI3K\/AKT pathway, which regulates cell survival, proliferation, and differentiation.\u003cbr\u003e\u003cbr\u003ePhospho-ErbB3 (Tyr1289) antibody is a critical reagent for studying growth factor receptor signaling. Aberrant activation of ErbB3 contributes to tumorigenesis and therapeutic resistance in cancers such as breast, ovarian, and gastric carcinoma. Elevated phosphorylation of ErbB3 is often associated with resistance to EGFR and HER2-targeted therapies, making detection of phospho-ErbB3 a valuable biomarker in oncology research.\u003cbr\u003e\u003cbr\u003eThe antibody is widely used in applications such as western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, Phospho-ErbB3 (Tyr1289) antibody distinguishes phosphorylated receptor isoforms from total ErbB3 protein, allowing researchers to quantify receptor activation states. In immunohistochemistry, it provides tissue-level localization of phosphorylated ErbB3, highlighting differences between normal and cancerous samples. Immunofluorescence enables visualization of receptor activation in cultured cells, often in combination with markers of proliferation or survival pathways.\u003cbr\u003e\u003cbr\u003ePhosphorylation at Tyr1289 plays a unique role in ErbB3 signaling because it directly recruits PI3K regulatory subunits, making this site essential for downstream signaling. Dysregulation of this phosphorylation leads to uncontrolled activation of the PI3K\/AKT pathway, promoting survival and growth in tumor cells. Studies employing Phospho-ErbB3 (Tyr1289) antibody have shown that inhibition of ErbB3 phosphorylation can sensitize resistant tumors to kinase inhibitors, emphasizing its translational relevance.\u003cbr\u003e\u003cbr\u003eBeyond oncology, ErbB3 signaling has been implicated in neural development and cardiac biology, where receptor tyrosine kinases regulate cell fate and tissue maintenance. The use of phospho-specific antibodies enables researchers to connect receptor activation with physiological and pathological outcomes in diverse tissues.\u003cbr\u003e\u003cbr\u003ePhospho-ErbB3 (Tyr1289) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ERBB3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ERBB3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ERBB3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ERBB3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ERBB3+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=ERBB3 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047285580141,"sku":"FY12312","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/phospho-erbb3-tyr1289-antibody-her3-bha17135215","provider":"BioHippo","version":"1.0","type":"link"}