{"product_id":"phospho-fak-tyr576-tyr577-antibody-focal-adhesion-kinase-1-bha17135475","title":"Phospho-FAK (Tyr576\/Tyr577) Antibody \/ Focal Adhesion Kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-FAK (Tyr576\/Tyr577) Antibody \/ Focal Adhesion Kinase 1 is a anti-FAK (phospho-Tyr576, Tyr577) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P46 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FAK (phospho site: Tyr576, Tyr577)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P46, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-FAK (Tyr576\/Tyr577) antibody detects focal adhesion kinase 1 when phosphorylated at tyrosine residues 576 and 577. Focal adhesion kinase, encoded by the PTK2 gene, is a cytoplasmic tyrosine kinase that regulates adhesion, migration, and survival signaling. Autophosphorylation at Tyr397 recruits Src family kinases, which subsequently phosphorylate Tyr576 and Tyr577 within the kinase activation loop. Phosphorylation at these residues enhances catalytic activity and promotes downstream signaling via MAPK and PI3K pathways.\u003cbr\u003e\u003cbr\u003ePhospho-FAK (Tyr576\/Tyr577) antibody is widely used in cancer biology, cell adhesion studies, and mechanotransduction research. Active FAK drives tumor progression, metastasis, and angiogenesis by promoting migration and survival of cancer cells. Detection of phosphorylation at Tyr576\/577 provides a direct measure of FAK activation and signaling output. By using this antibody, researchers can evaluate FAK driven pathways in oncogenesis, wound healing, and fibrosis.\u003cbr\u003e\u003cbr\u003e Western blot assays distinguish phosphorylated FAK from unmodified protein, while immunohistochemistry maps activation in tissues undergoing remodeling or tumor progression. Immunofluorescence highlights localization of phospho-FAK at focal adhesions, providing spatial insight into adhesion-dependent signaling events. These approaches allow comprehensive analysis of FAK activation in different systems.\u003cbr\u003e\u003cbr\u003ePhosphorylation at Tyr576 and Tyr577 is essential for full kinase activation. It promotes conformational changes that enhance substrate binding and signaling complex assembly. Dysregulated FAK phosphorylation contributes to cancer cell invasion and therapy resistance. By applying Phospho-FAK (Tyr576\/Tyr577) antibody, scientists can study therapeutic strategies that target adhesion-dependent kinase activity and its role in disease.\u003cbr\u003e\u003cbr\u003eFAK signaling also participates in cardiovascular and developmental biology, where it regulates vascular remodeling, stem cell adhesion, and tissue morphogenesis. Its integration of mechanical and biochemical signals makes it a central regulator of cell behavior. The phospho-specific antibody therefore serves as a powerful tool for examining mechanosensitive signaling networks.\u003cbr\u003e\u003cbr\u003ePhospho-FAK (Tyr576\/Tyr577) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FAK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FAK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FAK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FAK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FAK+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=FAK --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047292330349,"sku":"FY12572","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_19fde0ec-6f24-4b0f-8a96-06d361e65fda.jpg?v=1772019311","url":"https:\/\/www.ebiohippo.com\/products\/phospho-fak-tyr576-tyr577-antibody-focal-adhesion-kinase-1-bha17135475","provider":"BioHippo","version":"1.0","type":"link"}