{"product_id":"phospho-hist1h1e-s35-antibody-bha10532022","title":"Phospho-HIST1H1E (S35) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-HIST1H1E antibody raised in Rabbit, tested in ELISA, ICC, IF. Designed to detect HIST1H1E in Human for epigenetics and nuclear signaling research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Peptide sequence around site of Phospho-Ser (35) derived from Human Histone H1.4 — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eHIST1H1E\u003c\/strong\u003e (also: H1 histone family member 4, H1.4, H14_HUMAN, H1E, H1F4, Hist1h1e, Histone 1 H1e, Histone cluster 1 H1e, Histone H1, Histone H1.4, Histone H1B, MGC116819) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current HIST1H1E annotation relevant to epigenetics and nuclear signaling.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eICC:\u003c\/strong\u003e Applicable for ICC-based detection; optimize per documentation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIF:\u003c\/strong\u003e Subcellular localization in fixed cells\/sections; optimize fixation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"50 uL","offer_id":53241123471725,"sku":"CSB-PA010380PA35phHU-50UL","price":175.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53241214828909,"sku":"CSB-PA010380PA35phHU-100UL","price":319.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA010380PA35phHU-ICC.jpg?v=1776692893","url":"https:\/\/www.ebiohippo.com\/products\/phospho-hist1h1e-s35-antibody-bha10532022","provider":"BioHippo","version":"1.0","type":"link"}