{"product_id":"phospho-ikb-alpha-tyr42-antibody-bha17135648","title":"Phospho-IKB alpha (Tyr42) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-IKB alpha (Tyr42) Antibody is a anti-IKB (phospho-Tyr42) Rabbit antibody Recombinant Rabbit Monoclonal clone 32N56 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IKB (phospho site: Tyr42)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32N56, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-IKB alpha (Tyr42) antibody detects inhibitor of kappa B alpha when phosphorylated at tyrosine 42. This protein, encoded by the NFKBIA gene, is also referred to as NF kappa B inhibitor alpha, MAD3, and IKBA. IKB alpha is a member of the I kappa B protein family, which functions as central regulators of NF kappa B transcription factors. In resting cells, IKB alpha binds NF kappa B dimers such as p65 and p50, sequestering them in the cytoplasm and preventing transcription of inflammatory and survival genes. Phosphorylation at Tyr42 represents a non canonical regulatory event that differs from the classical serine phosphorylation that targets IKB alpha for degradation.\u003cbr\u003e\u003cbr\u003ePhospho-IKB alpha (Tyr42) antibody is applied widely in immunology, cancer biology, and signal transduction research. NF kappa B signaling is one of the most important regulators of immunity, inflammation, and cell survival. Detecting Tyr42 phosphorylation allows researchers to distinguish oxidative stress induced NF kappa B activation from canonical TNF and IL1 mediated activation. This distinction is critical for studies dissecting how different stimuli converge on the NF kappa B pathway.\u003cbr\u003e\u003cbr\u003ePhosphorylation at Tyr42 is mediated by kinases such as c Src, Syk, and other non receptor tyrosine kinases. Unlike serine phosphorylation at Ser32 and Ser36, which leads to ubiquitination and degradation of IKB alpha, phosphorylation at Tyr42 can stabilize IKB alpha or change its interactions with NF kappa B subunits. This dual regulatory system provides cells with flexibility to tailor transcriptional responses under stress, infection, or DNA damage. Phospho-IKB alpha (Tyr42) antibody enables researchers to track these unique signaling events.\u003cbr\u003e\u003cbr\u003eApplications of this antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays distinguish phosphorylated IKB alpha bands from non modified protein, providing direct evidence of signaling activation. Immunohistochemistry reveals spatial distribution of Tyr42 phosphorylation in tissues such as inflamed mucosa or tumor biopsies. Immunofluorescence detects dynamic changes in subcellular localization, showing how NF kappa B and IKB alpha shuttle between cytoplasm and nucleus after stimulation. These techniques allow mechanistic dissection of cell signaling in both cultured cells and patient samples.\u003cbr\u003e\u003cbr\u003eThe biological consequences of Tyr42 phosphorylation are complex. In some contexts, phosphorylation promotes apoptosis by releasing NF kappa B from IKB alpha, while in others it stabilizes IKB alpha and suppresses NF kappa B activity. This duality underscores the importance of site specific phospho antibodies. By applying Phospho-IKB alpha (Tyr42) antibody, researchers can resolve conflicting mechanisms and generate precise models of NF kappa B regulation.\u003cbr\u003e\u003cbr\u003eAberrant NF kappa B signaling contributes to oncogenesis, autoimmune disease, and chronic inflammatory disorders. Phosphorylation at Tyr42 has been detected in leukemias, lymphomas, and solid tumors, where altered IKB alpha activity influences tumor cell survival and resistance to therapy. Monitoring this modification with antibody based assays can provide biomarkers for patient stratification and therapeutic development.\u003cbr\u003e\u003cbr\u003eIn addition to cancer, Tyr42 phosphorylation has been linked to oxidative stress responses in cardiovascular disease, neurodegeneration, and aging. For example, ischemic heart tissue shows altered IKB alpha phosphorylation patterns, while neurons under oxidative stress exhibit abnormal NF kappa B signaling that contributes to cell death. Phospho-IKB alpha (Tyr42) antibody therefore has applications extending beyond immunology into metabolic and neurological research.\u003cbr\u003e\u003cbr\u003ePhospho-IKB alpha (Tyr42) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=IKB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=IKB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=IKB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/IKB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=IKB+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=IKB --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047298425197,"sku":"FY12745","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/phospho-ikb-alpha-tyr42-antibody-bha17135648","provider":"BioHippo","version":"1.0","type":"link"}