{"product_id":"phospho-nucleophosmin-ser125-antibody-npm1-bha17135912","title":"Phospho-Nucleophosmin (Ser125) Antibody \/ NPM1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Nucleophosmin (Ser125) Antibody \/ NPM1 is a anti-NPM1 (phospho-Ser125) Rabbit antibody Recombinant Rabbit Monoclonal clone 32N71 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NPM1 (phospho site: Ser125)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32N71, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Nucleophosmin (Ser125) antibody detects Nucleophosmin when phosphorylated at serine 125, encoded by the NPM1 gene. Nucleophosmin is a multifunctional phosphoprotein that shuttles between the nucleolus, nucleus, and cytoplasm. It is involved in ribosome biogenesis, centrosome duplication, DNA repair, and regulation of tumor suppressors such as ARF and p53. Phosphorylation of Nucleophosmin at serine 125 plays a critical role in controlling its subcellular localization and activity, making Phospho-Nucleophosmin (Ser125) antibody a valuable reagent for studies of cell cycle progression, oncogenesis, and stress response.\u003cbr\u003e\u003cbr\u003eNucleophosmin is abundant in the nucleolus, where it functions in ribosomal RNA processing and assembly of ribosomal subunits. Phosphorylation at serine 125 occurs during mitosis, and research with Phospho-Nucleophosmin (Ser125) antibody has revealed that this modification promotes centrosome separation and spindle formation. By regulating Nucleophosmin function, phosphorylation ensures faithful chromosome segregation and prevents aneuploidy. Disruption of this modification impairs mitotic progression and contributes to genomic instability, highlighting its importance in cell division control.\u003cbr\u003e\u003cbr\u003eNPM1 is also a key player in hematological malignancies. Mutations in NPM1, particularly frame-shift mutations in exon 12, are among the most frequent genetic alterations in acute myeloid leukemia. These mutations cause aberrant cytoplasmic localization of Nucleophosmin, altering its regulatory functions. Research using Phospho-Nucleophosmin (Ser125) antibody has shown that phosphorylation state influences localization and protein interactions, potentially modulating leukemogenesis. Furthermore, phosphorylation may interact with mutational effects, making it an area of interest in understanding leukemia biology and therapy.\u003cbr\u003e\u003cbr\u003eBeyond cancer, Nucleophosmin phosphorylation contributes to stress responses, DNA repair, and apoptosis regulation. Phospho-Nucleophosmin (Ser125) antibody has been used to demonstrate that cellular stress induces phosphorylation at this site, altering interactions with ARF and p53. These findings suggest a broader role for Nucleophosmin phosphorylation in tumor suppression and cell fate decisions. Since Nucleophosmin participates in chromatin remodeling, this modification may also influence transcription and epigenetic regulation.\u003cbr\u003e\u003cbr\u003ePhospho-Nucleophosmin (Ser125) antibody is widely applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting distinguishes phosphorylated from unmodified forms, while immunofluorescence visualizes dynamic localization changes during the cell cycle. Immunohistochemistry allows detection of phosphorylation patterns in tumor tissue, linking biochemical modification to disease phenotype. These experimental approaches provide insights into the regulation of Nucleophosmin in health and disease.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-Nucleophosmin (Ser125) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NPM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NPM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NPM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NPM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NPM1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=NPM1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308878189,"sku":"FY13010","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0d5dc535-a4bc-4630-a0d9-a7d5359bfc38.jpg?v=1772019378","url":"https:\/\/www.ebiohippo.com\/products\/phospho-nucleophosmin-ser125-antibody-npm1-bha17135912","provider":"BioHippo","version":"1.0","type":"link"}