{"product_id":"phospho-p120-catenin-tyr228-antibody-ctnnd1-bha17136214","title":"Phospho-p120 Catenin (Tyr228) Antibody \/ CTNND1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-p120 Catenin (Tyr228) Antibody \/ CTNND1 is a anti-P120 (phospho-Tyr228) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C33 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e P120 (phospho site: Tyr228)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C33, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-p120 Catenin (Tyr228) antibody detects p120 Catenin phosphorylated at tyrosine 228, encoded by the CTNND1 gene. p120 Catenin is an armadillo repeat protein that functions as a critical regulator of cell-cell adhesion, cytoskeletal organization, and intracellular signaling. Phosphorylation at tyrosine 228 is an important post-translational modification that influences its interaction with cadherins, small GTPases, and signaling pathways. Phospho-p120 Catenin (Tyr228) antibody provides researchers with a highly specific reagent for dissecting the regulation of adherens junctions and downstream signaling events.\u003cbr\u003e\u003cbr\u003ep120 Catenin was first identified as a substrate of Src family kinases and is now recognized as a central adaptor in adherens junctions, where it binds directly to the juxtamembrane domain of classical cadherins. Research using Phospho-p120 Catenin (Tyr228) antibody has shown that phosphorylation at Tyr228 modifies its cadherin-binding affinity, affecting junction stability and turnover. This phosphorylation also alters signaling through Rho family GTPases, impacting actin cytoskeleton dynamics and cell motility.\u003cbr\u003e\u003cbr\u003eStudies with Phospho-p120 Catenin (Tyr228) antibody have revealed that the Tyr228 site is phosphorylated in response to growth factor stimulation, mechanical stress, and oncogenic signaling. Src and receptor tyrosine kinases mediate this modification, linking extracellular cues to junction remodeling. By regulating cell-cell adhesion, phosphorylation at Tyr228 influences epithelial-mesenchymal transition, tissue morphogenesis, and tumor invasion.\u003cbr\u003e\u003cbr\u003eIn cancer biology, aberrant phosphorylation of p120 Catenin contributes to disease progression. Research using Phospho-p120 Catenin (Tyr228) antibody has shown that elevated phosphorylation disrupts cadherin-mediated adhesion, enabling tumor cells to detach and migrate. At the same time, phosphorylation-dependent regulation of Rho family proteins promotes cytoskeletal reorganization and invasiveness. These findings highlight p120 Catenin Tyr228 phosphorylation as a potential biomarker of metastasis and therapeutic target.\u003cbr\u003e\u003cbr\u003eBeyond cancer, p120 Catenin phosphorylation influences cardiovascular and developmental processes. Studies with Phospho-p120 Catenin (Tyr228) antibody have revealed roles in vascular integrity, cardiac morphogenesis, and neural crest development. Dysregulation of this pathway may contribute to congenital defects and vascular diseases. Because p120 Catenin integrates adhesive and signaling functions, its phosphorylation status provides insight into multiple physiological and pathological processes.\u003cbr\u003e\u003cbr\u003ePhospho-p120 Catenin (Tyr228) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated from non-phosphorylated forms, immunohistochemistry identifies activated signaling regions in tissues, and immunofluorescence demonstrates subcellular distribution of phosphorylated protein at junctions and in the cytoplasm. These applications make the antibody valuable in adhesion and signaling research.\u003cbr\u003e\u003cbr\u003eBy providing validated Phospho-p120 Catenin (Tyr228) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=P120 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=P120 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=P120 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/P120 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=P120+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=P120 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322214765,"sku":"FY13312","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_af77c286-ea97-49ea-a9c7-389f13f3933b.jpg?v=1772019425","url":"https:\/\/www.ebiohippo.com\/products\/phospho-p120-catenin-tyr228-antibody-ctnnd1-bha17136214","provider":"BioHippo","version":"1.0","type":"link"}