{"product_id":"phospho-p38-thr180-antibody-mapk14-bha17135893","title":"Phospho-p38 (Thr180) Antibody \/ MAPK14","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-p38 (Thr180) Antibody \/ MAPK14 is a anti-P38 (phospho-Thr180) Rabbit antibody Recombinant Rabbit Monoclonal clone 31M76 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e P38 (phospho site: Thr180)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31M76, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-p38 (Thr180) antibody detects p38 mitogen activated protein kinase when phosphorylated at threonine 180. The protein is encoded by the MAPK14 gene and is a key member of the stress activated MAP kinase family. Activation of p38 occurs through dual phosphorylation at threonine 180 and tyrosine 182 by upstream kinases such as MKK3 and MKK6. Phospho-p38 (Thr180) antibody provides a specific reagent to monitor pathway activation during responses to cytokines, ultraviolet radiation, osmotic stress, and inflammatory signals.\u003cbr\u003e\u003cbr\u003ep38 mitogen activated protein kinase regulates a wide range of biological processes, including transcription, cell cycle arrest, apoptosis, and cytokine production. Following phosphorylation at threonine 180, p38 phosphorylates substrates such as ATF2, MAPKAPK2, and HSP27, linking stress signals to gene expression and cytoskeletal remodeling. Studies with Phospho-p38 (Thr180) antibody have shown that pathway activation is transient and tightly controlled, ensuring that cellular stress responses are adaptive rather than destructive. Dysregulation of this phosphorylation event contributes to inflammatory disease, cancer, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eIn immune biology, p38 activation promotes the production of cytokines such as TNF alpha, IL6, and IL1 beta. Research using Phospho-p38 (Thr180) antibody has demonstrated that inhibition of p38 phosphorylation reduces inflammatory cytokine output, supporting therapeutic strategies targeting this pathway. In oncology, altered p38 signaling influences tumor cell apoptosis, angiogenesis, and metastasis, making it a subject of active drug development. In neurons, aberrant phosphorylation contributes to synaptic dysfunction and degenerative disease.\u003cbr\u003e\u003cbr\u003ePhospho-p38 (Thr180) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals activation status across experimental conditions, while immunohistochemistry highlights tissue specific phosphorylation patterns. Immunofluorescence demonstrates subcellular distribution of active kinase in stressed cells. These applications provide critical insights into stress signaling, immune regulation, and pathological responses.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=P38 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=P38 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=P38 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/P38 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=P38+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=P38 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308288365,"sku":"FY12991","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bbb71c37-752d-4d13-a4bb-89bdad52a951.jpg?v=1772019374","url":"https:\/\/www.ebiohippo.com\/products\/phospho-p38-thr180-antibody-mapk14-bha17135893","provider":"BioHippo","version":"1.0","type":"link"}