{"product_id":"phospho-pbk-thr9-antibody-pdz-binding-kinase-bha17135695","title":"Phospho-PBK (Thr9) Antibody \/ PDZ binding kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-PBK (Thr9) Antibody \/ PDZ binding kinase is a anti-PBK (phospho-Thr9) Rabbit antibody Recombinant Rabbit Monoclonal clone 30P33 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PBK (phospho site: Thr9)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30P33, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-PBK (Thr9) antibody detects PDZ binding kinase, also known as lymphokine activated killer T cell origin protein kinase (TOPK), when phosphorylated at threonine 9. PBK is encoded by the PBK gene and belongs to the MAP kinase kinase family. It is highly expressed in proliferating cells, especially in testis, placenta, and activated lymphocytes. Phosphorylation at Thr9 regulates PBK kinase activity, influencing cell cycle progression and mitotic events. This makes Phospho-PBK (Thr9) antibody a key tool for studying proliferative signaling and tumor biology.\u003cbr\u003e\u003cbr\u003ePhospho-PBK (Thr9) antibody is widely applied in oncology, immunology, and cell cycle research. PBK activation supports G2\/M transition by phosphorylating downstream substrates involved in spindle formation and chromosome segregation. It also participates in immune signaling pathways in T cells and NK cells. By detecting phosphorylation at Thr9, researchers can monitor PBK activation and its role in proliferation and immunity.\u003cbr\u003e\u003cbr\u003eApplications for Phospho-PBK (Thr9) antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect phosphorylated PBK isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence reveals subcellular localization at centrosomes during mitosis. These complementary approaches provide detailed evaluation of PBK biology.\u003cbr\u003e\u003cbr\u003eDysregulated PBK activity is implicated in multiple cancers, including lung, breast, and hematologic malignancies. Elevated Thr9 phosphorylation correlates with tumor grade and poor prognosis, reflecting hyperactive proliferation. In immune biology, PBK phosphorylation regulates lymphocyte activation, linking cell cycle control to host defense. By applying Phospho-PBK (Thr9) antibody, scientists can study these dual roles in cancer and immunity.\u003cbr\u003e\u003cbr\u003eTherapeutic research has targeted PBK as a potential kinase for inhibition. Small molecule inhibitors reduce proliferation and sensitize tumor cells to chemotherapy. Monitoring Thr9 phosphorylation provides a pharmacodynamic readout of drug activity. Phospho-PBK (Thr9) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PBK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PBK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PBK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PBK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PBK+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PBK --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047299866989,"sku":"FY12793","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d8ae671c-4804-4321-a399-bfcef4d73bbd.jpg?v=1772019346","url":"https:\/\/www.ebiohippo.com\/products\/phospho-pbk-thr9-antibody-pdz-binding-kinase-bha17135695","provider":"BioHippo","version":"1.0","type":"link"}