{"product_id":"phospho-phospholamban-s16-antibody-pln-bha17135724","title":"Phospho-Phospholamban (S16) Antibody \/ PLN","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Phospholamban (S16) Antibody \/ PLN is a anti-PLN (phospho-Ser16) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P55 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLN (phospho site: Ser16)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P55, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Phospholamban (S16) antibody detects PHOSPHOLAMBAN, encoded by the PLN gene, when phosphorylated at serine 16. Alternate names include cardiac phospholamban, PLB, and phospholamban phosphoprotein. PHOSPHOLAMBAN is a small transmembrane protein in the sarcoplasmic reticulum of cardiac and skeletal muscle. It regulates SERCA2a, the sarcoplasmic reticulum Ca2+ ATPase responsible for calcium uptake during muscle relaxation. In its unphosphorylated state, PHOSPHOLAMBAN inhibits SERCA2a. Phosphorylation at serine 16 by PKA relieves this inhibition, enhancing calcium uptake, promoting relaxation, and increasing cardiac contractility.\u003cbr\u003e\u003cbr\u003ePhospho-Phospholamban (S16) antibody is widely applied in cardiovascular biology, muscle physiology, and signaling research. By detecting phosphorylation at serine 16, researchers can study how beta adrenergic signaling enhances cardiac output through PKA mediated regulation of calcium cycling. This site specific antibody allows distinction between active and inhibited PHOSPHOLAMBAN, providing insights into heart function under physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003eApplications include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated PHOSPHOLAMBAN in heart lysates, immunohistochemistry maps phosphorylation in cardiac tissue sections, and immunofluorescence highlights localization in sarcoplasmic reticulum. ELISA assays allow quantification of phosphorylation status in experimental models. These techniques provide direct evidence of cardiac signaling events.\u003cbr\u003e\u003cbr\u003eDysregulation of PHOSPHOLAMBAN phosphorylation contributes to heart failure, arrhythmias, and cardiomyopathies. Mutations in PLN cause inherited dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy. Monitoring serine 16 phosphorylation provides biomarkers for disease progression and therapy efficacy. By applying Phospho-Phospholamban (S16) antibody, scientists can investigate how altered signaling leads to impaired cardiac relaxation and contractility.\u003cbr\u003e\u003cbr\u003ePhosphorylation of PHOSPHOLAMBAN is also studied in exercise physiology, where beta adrenergic stimulation enhances contractility. The antibody supports research into how training, drugs, and genetic variants influence calcium cycling. Translational applications include evaluation of therapeutic interventions targeting SERCA2a regulation, such as gene therapy or pharmacologic modulators.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLN+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PLN --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047302816109,"sku":"FY12822","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_853235f1-2255-49f5-b7ab-d788340d6314.jpg?v=1772019349","url":"https:\/\/www.ebiohippo.com\/products\/phospho-phospholamban-s16-antibody-pln-bha17135724","provider":"BioHippo","version":"1.0","type":"link"}