{"product_id":"phospho-pkc-alpha-thr497-antibody-protein-kinase-c-alpha-bha17135306","title":"Phospho-PKC alpha (Thr497) Antibody \/ Protein kinase C alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-PKC alpha (Thr497) Antibody \/ Protein kinase C alpha is a anti-PKC (phospho-Thr497) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P00 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKC (phospho site: Thr497)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P00, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-PKC alpha Thr497 antibody recognizes protein kinase C alpha phosphorylated at threonine 497, an activation site essential for catalytic function. PKC alpha is a member of the conventional protein kinase C family that functions as a serine threonine kinase. It is activated by calcium, diacylglycerol, and phospholipids, and it regulates processes including cell proliferation, differentiation, apoptosis, and migration. Phosphorylation at Thr497 stabilizes the kinase in an active conformation and promotes downstream substrate phosphorylation.\u003cbr\u003e\u003cbr\u003ePhospho-PKC alpha Thr497 antibody is widely applied in signaling and phosphorylation research. By distinguishing the phosphorylated active form from inactive PKC alpha, it enables precise monitoring of pathway activation. This is particularly relevant in studies of cancer, cardiovascular disease, and neuronal signaling where PKC alpha activity is tightly regulated.\u003cbr\u003e\u003cbr\u003eThe antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC alpha Thr497 antibody detects phosphorylated isoforms and allows quantification of activation under various stimuli. In immunohistochemistry, it highlights phosphorylation dependent localization within tissues, while immunofluorescence provides single cell resolution of active PKC alpha.\u003cbr\u003e\u003cbr\u003ePKC alpha has been implicated in cancer progression, where dysregulated activation contributes to survival, proliferation, and metastasis. Monitoring Thr497 phosphorylation with phospho specific antibodies provides mechanistic insight into tumor biology and therapeutic targeting. In cardiovascular research, PKC alpha activity is linked to hypertrophy, contractility, and vascular remodeling. In the nervous system, PKC alpha influences synaptic plasticity and long term potentiation.\u003cbr\u003e\u003cbr\u003ePhospho-PKC alpha Thr497 antibody offered by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PKC --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047287939437,"sku":"FY12403","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2f3034e1-291f-448d-832b-f11f8fefa58e.jpg?v=1772019281","url":"https:\/\/www.ebiohippo.com\/products\/phospho-pkc-alpha-thr497-antibody-protein-kinase-c-alpha-bha17135306","provider":"BioHippo","version":"1.0","type":"link"}