{"product_id":"phospho-pkc-delta-thr507-antibody-protein-kinase-c-delta-bha17135289","title":"Phospho-PKC delta (Thr507) Antibody \/ Protein kinase C delta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-PKC delta (Thr507) Antibody \/ Protein kinase C delta is a anti-PKC (phospho-Thr507) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P74 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKC (phospho site: Thr507)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P74, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-PKC delta Thr507 antibody recognizes protein kinase C delta phosphorylated at threonine 507. PKC delta is a member of the novel protein kinase C subfamily that functions as a serine threonine kinase involved in diverse cellular processes. Phosphorylation at threonine 507 within the activation loop is essential for catalytic activity and downstream signaling. This modification stabilizes the kinase conformation and enables phosphorylation of target proteins involved in proliferation, apoptosis, and immune responses.\u003cbr\u003e\u003cbr\u003ePhospho-PKC delta Thr507 antibody is widely used to study kinase signaling and phosphorylation dependent regulation. By specifically detecting PKC delta in its activated phosphorylated form, the antibody allows researchers to distinguish between inactive and active signaling states. This is particularly important in studies of cancer, cardiovascular disease, and inflammation, where PKC delta activity is tightly regulated and often dysregulated.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC delta Thr507 antibody detects phosphorylated isoforms of the kinase, enabling quantification of activation under different treatments. In immunohistochemistry, it reveals phosphorylation dependent localization within tissues, highlighting regions of signaling activation. Immunofluorescence experiments allow visualization of PKC delta phosphorylation at the single cell level, providing insight into dynamic regulatory events.\u003cbr\u003e\u003cbr\u003ePKC delta has been implicated in pro apoptotic signaling pathways, where activation at Thr507 contributes to programmed cell death. Conversely, in certain cancer contexts, PKC delta activity promotes survival and proliferation. Monitoring Thr507 phosphorylation using phospho specific antibodies provides mechanistic understanding of how PKC delta signaling outcomes vary depending on cellular context. In cardiovascular biology, PKC delta contributes to ischemia reperfusion injury and vascular remodeling, making its phosphorylation status a useful biomarker of stress responses.\u003cbr\u003e\u003cbr\u003ePhospho-PKC delta Thr507 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PKC --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047287382381,"sku":"FY12386","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/phospho-pkc-delta-thr507-antibody-protein-kinase-c-delta-bha17135289","provider":"BioHippo","version":"1.0","type":"link"}