{"product_id":"phospho-pkc-delta-tyr311-antibody-prkcd-bha17136276","title":"Phospho-PKC delta (Tyr311) Antibody \/ PRKCD","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-PKC delta (Tyr311) Antibody \/ PRKCD is a anti-PKC (phospho-Tyr311) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P27 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKC (phospho site: Tyr311)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P27, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-PKC delta (Tyr311) antibody detects PKC delta phosphorylated at tyrosine 311, encoded by the PRKCD gene. PKC delta is a member of the novel protein kinase C family of serine-threonine kinases, which are activated by diacylglycerol but not by calcium. Phosphorylation at Tyr311 is a critical regulatory modification that enhances PKC delta catalytic activity and determines substrate specificity. Phospho-PKC delta (Tyr311) antibody provides researchers with a precise tool to study how tyrosine phosphorylation integrates into PKC signaling, apoptosis regulation, and stress responses.\u003cbr\u003e\u003cbr\u003ePKC delta is widely expressed and participates in multiple signaling pathways. Research using Phospho-PKC delta (Tyr311) antibody has shown that phosphorylation at Tyr311 occurs downstream of growth factor receptors, integrin engagement, and stress-inducing stimuli such as DNA damage and oxidative stress. This modification is catalyzed by upstream kinases including Src family kinases and Abl, which directly phosphorylate PKC delta on its regulatory domain. Phosphorylation at Tyr311 alters the conformation of PKC delta, promoting kinase activity and enabling interaction with specific substrates. These findings illustrate the importance of Tyr311 phosphorylation in fine-tuning PKC delta signaling output.\u003cbr\u003e\u003cbr\u003eOne of the major biological roles of PKC delta phosphorylated at Tyr311 is regulation of apoptosis. Studies with Phospho-PKC delta (Tyr311) antibody have revealed that this modification supports pro-apoptotic signaling in response to genotoxic stress. PKC delta phosphorylated at Tyr311 translocates to mitochondria, where it interacts with Bcl-2 family proteins to promote cytochrome c release and caspase activation. In parallel, it phosphorylates nuclear substrates to enhance DNA fragmentation and apoptosis execution. This dual mitochondrial and nuclear activity underscores PKC delta's role as a stress-activated pro-death kinase.\u003cbr\u003e\u003cbr\u003eIn cancer research, PKC delta shows context-dependent functions. Research using Phospho-PKC delta (Tyr311) antibody has shown that in certain malignancies, such as glioblastoma and breast cancer, PKC delta acts as a tumor suppressor by promoting apoptosis through Tyr311 phosphorylation. Conversely, in other cancers, PKC delta activity supports survival and migration. The specific role depends on cellular context, phosphorylation status, and cross-talk with other signaling pathways. These observations highlight the complexity of targeting PKC delta in oncology.\u003cbr\u003e\u003cbr\u003eCardiovascular studies have also linked PKC delta phosphorylation at Tyr311 to heart disease. Research using Phospho-PKC delta (Tyr311) antibody has demonstrated that excessive activation promotes cardiomyocyte apoptosis during ischemia-reperfusion injury, contributing to loss of viable myocardium. Similarly, PKC delta regulates vascular smooth muscle contraction and remodeling. Dysregulation of Tyr311 phosphorylation has been associated with atherosclerosis and vascular dysfunction, suggesting a role for PKC delta in cardiovascular pathology.\u003cbr\u003e\u003cbr\u003eBeyond apoptosis and cardiovascular disease, PKC delta is involved in immune signaling and inflammation. Studies with Phospho-PKC delta (Tyr311) antibody have revealed that Tyr311 phosphorylation modulates cytokine production, macrophage activation, and antigen receptor signaling. By influencing immune cell function, PKC delta participates in host defense as well as chronic inflammatory conditions. These findings expand its functional repertoire beyond classical stress and apoptosis pathways.\u003cbr\u003e\u003cbr\u003ePhospho-PKC delta (Tyr311) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. In western blotting, it detects the phosphorylated form of PKC delta as distinct from total protein levels, providing a readout of activation state. Immunohistochemistry highlights tissue-specific regions of PKC delta activation, including tumors, heart, and vascular tissue under stress conditions. Immunofluorescence shows subcellular distribution, often revealing mitochondrial or nuclear localization in apoptotic cells. Together, these applications demonstrate the utility of the antibody in both basic science and translational research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-PKC delta (Tyr311) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PKC --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047323885933,"sku":"FY13374","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3cb45ef7-7380-496a-8324-b1da2d97cb71.jpg?v=1772019436","url":"https:\/\/www.ebiohippo.com\/products\/phospho-pkc-delta-tyr311-antibody-prkcd-bha17136276","provider":"BioHippo","version":"1.0","type":"link"}