{"product_id":"phospho-pkc-ser729-antibody-prkca-prkcb-prkce-bha17135586","title":"Phospho-PKC (Ser729) Antibody \/ PRKCA\/PRKCB\/PRKCE","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-PKC (Ser729) Antibody \/ PRKCA\/PRKCB\/PRKCE is a anti-PKC (phospho-Ser729) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P75 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKC (phospho site: Ser729)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P75, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-PKC (Ser729) antibody detects protein kinase C when phosphorylated at serine 729. Protein kinase C belongs to a family of serine threonine kinases encoded by multiple genes including PRKCA, PRKCB, and PRKCG. Phosphorylation at Ser729 occurs within the hydrophobic motif of PKC alpha and related isoforms, a modification essential for stability and full catalytic activity. This phosphorylation is typically mediated by kinases such as PDK1 and mTORC2, which regulate PKC maturation and downstream signaling capacity.\u003cbr\u003e\u003cbr\u003ePhospho-PKC (Ser729) antibody is widely applied in cancer biology, signal transduction, and neuroscience. PKC enzymes regulate cell proliferation, differentiation, apoptosis, and cytoskeletal remodeling. Phosphorylation at Ser729 serves as a marker for mature active PKC isoforms and is required for proper subcellular localization and substrate phosphorylation. By detecting phospho Ser729, researchers can measure activation status of PKC pathways and how they change in disease states.\u003cbr\u003e\u003cbr\u003e Western blot assays reveal distinct phospho PKC bands corresponding to activated isoforms. Immunohistochemistry maps Ser729 phosphorylation in tissues such as brain, heart, and tumors. Immunofluorescence highlights dynamic translocation of PKC from cytosol to plasma membrane or other compartments upon stimulation. These methods allow precise characterization of PKC activation at the molecular and cellular level.\u003cbr\u003e\u003cbr\u003ePKC activity is critical for oncogenic signaling. Dysregulation of phosphorylation at Ser729 is associated with altered PKC stability and oncogenic transformation. Certain cancers show reduced PKC activity due to mutations preventing proper phosphorylation, while others display hyperactivation that promotes tumor growth. By applying Phospho-PKC (Ser729) antibody, scientists can study how phosphorylation events regulate tumor biology and therapeutic response.\u003cbr\u003e\u003cbr\u003ePKC enzymes are also central to neuronal signaling and plasticity. Phosphorylation at Ser729 supports synaptic transmission, long term potentiation, and memory formation. Altered PKC activity has been implicated in neurodegenerative diseases such as Alzheimer disease and Parkinson disease. With phospho specific antibody tools, researchers can dissect how PKC phosphorylation influences brain function and pathology.\u003cbr\u003e\u003cbr\u003eIn cardiology, PKC phosphorylation regulates cardiac hypertrophy, contractility, and response to ischemia. The Ser729 site provides a biomarker for assessing PKC function in cardiac stress and adaptation. This makes Phospho-PKC (Ser729) antibody relevant not only for molecular signaling studies but also for translational research into heart disease.\u003cbr\u003e\u003cbr\u003ePhospho-PKC (Ser729) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=PKC --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297278317,"sku":"FY12683","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_808a8f39-bb83-4444-9001-cfe9c152d32b.jpg?v=1772019329","url":"https:\/\/www.ebiohippo.com\/products\/phospho-pkc-ser729-antibody-prkca-prkcb-prkce-bha17135586","provider":"BioHippo","version":"1.0","type":"link"}