{"product_id":"phospho-rapgef1-y504-antibody-bha10539996","title":"Phospho-RAPGEF1 (Y504) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-RAPGEF1 antibody raised in Rabbit, with confirmed utility in WB, ELISA. It is designed to detect RAPGEF1 protein in Human and supports researchers working in signal transduction contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthesized peptide derived from Human C3G around the phosphorylation site of Y504. — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRAPGEF1\u003c\/strong\u003e (also referred to as C3G, C3G protein, CRK SH3 binding GNRP, CRK SH3-binding GNRP, DKFZp781P1719, GRF 2, GRF2, Guanine nucleotide releasing factor 2 (specific for crk proto oncogene), Guanine nucleotide releasing factor 2, Guanine nucleotide-releasing factor 2, OTTHUMP00000064558, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, Rap guanine nucleotide exchange factor 1, RAPGEF 1, RAPGEF1, RPGF1_HUMAN) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RAPGEF1 biology, including known isoforms, interactors, and disease-relevant expression patterns in signal transduction.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e Western blot confirms target molecular weight and distinguishes isoforms under denaturing conditions. Expected band size should align with reported MW.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53245851828589,"sku":"CSB-PA008953-50UG","price":122.57,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53245944070509,"sku":"CSB-PA008953-100UG","price":204.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_fda8e853-bcd0-4a11-a22a-c479da43d3f5.jpg?v=1784927325","url":"https:\/\/www.ebiohippo.com\/products\/phospho-rapgef1-y504-antibody-bha10539996","provider":"BioHippo","version":"1.0","type":"link"}