{"product_id":"phospho-rasgrf1-ser916-antibody-bha10560165","title":"Phospho-RASGRF1 (Ser916) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-RASGRF1 antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect RASGRF1 protein in Mouse, Rat and supports researchers working in signal transduction contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1. — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRASGRF1\u003c\/strong\u003e (also referred to as CDC25, CDC25L, GNRP, GRF1, GRF55, Guanine nucleotide exchange factor, Guanine nucleotide releasing factor 55 kD, Guanine nucleotide releasing protein, guanine nucleotide-releasing factor 1, Guanine nucleotide-releasing protein, H GRF55, HGRF55, PP13187, Ras protein specific guanine nucleotide releasing factor 1, Ras specific guanine nucleotide releasing factor, Ras specific guanine nucleotide releasing factor CDC25 homolog, Ras specific nucleotide exchange factor CDC25, Ras-GRF1, Ras-specific guanine nucleotide-releasing factor 1, Ras-specific nucleotide exchange factor CDC25, RASGRF1, RGRF1_HUMAN) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of RASGRF1 biology in signal transduction.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Mouse, Rat. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"100 uL","offer_id":53247861260653,"sku":"CSB-PA256747-100UL","price":360.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA256747-IHC-1.jpg?v=1776792453","url":"https:\/\/www.ebiohippo.com\/products\/phospho-rasgrf1-ser916-antibody-bha10560165","provider":"BioHippo","version":"1.0","type":"link"}