{"product_id":"phospho-rb-ser780-antibody-retinoblastoma-bha17136200","title":"Phospho-Rb (Ser780) Antibody \/ Retinoblastoma","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Rb (Ser780) Antibody \/ Retinoblastoma is a anti-Rb (phospho-Ser780) Rabbit antibody Recombinant Rabbit Monoclonal clone 31R44 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Rb (phospho site: Ser780)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31R44, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Rb (Ser780) antibody detects Retinoblastoma associated protein phosphorylated at serine 780, encoded by the RB1 gene. Retinoblastoma associated protein is a tumor suppressor that regulates the cell cycle by controlling E2F transcription factors. Phosphorylation at serine 780 represents one of several regulatory modifications that control its activity. Phospho-Rb (Ser780) antibody provides researchers with a powerful tool for investigating cell cycle regulation, tumor biology, and signaling pathways.\u003cbr\u003e\u003cbr\u003eRetinoblastoma associated protein functions as a gatekeeper of the G1 to S phase transition. Research using Phospho-Rb (Ser780) antibody has shown that phosphorylation at serine 780, mediated by cyclin dependent kinases such as CDK4 and CDK6, inactivates its ability to bind E2F transcription factors. This releases E2F, allowing transcription of genes required for DNA synthesis. Thus, phosphorylation at Ser780 is a critical step in promoting cell cycle progression.\u003cbr\u003e\u003cbr\u003eStudies with Phospho-Rb (Ser780) antibody have revealed that this modification integrates growth factor signals with cell cycle machinery. In proliferating cells, growth signals activate cyclin D\/CDK4 complexes, which phosphorylate Rb at serine 780. In contrast, in quiescent cells or under growth arrest conditions, Rb remains hypophosphorylated and bound to E2F, maintaining transcriptional repression. This phosphorylation-dependent switch ensures precise regulation of proliferation.\u003cbr\u003e\u003cbr\u003eDysregulation of Retinoblastoma associated protein phosphorylation contributes to cancer. Research using Phospho-Rb (Ser780) antibody has demonstrated that hyperphosphorylation leads to loss of tumor suppressor function, uncontrolled cell cycle entry, and oncogenic transformation. Mutations or deletions of RB1 similarly disrupt its ability to restrain E2F activity, resulting in tumorigenesis. Monitoring phosphorylation at Ser780 provides critical information about cell cycle deregulation in cancer.\u003cbr\u003e\u003cbr\u003ePhospho-Rb (Ser780) antibody is widely used in western blotting, immunohistochemistry, and flow cytometry. Western blotting distinguishes phosphorylated from unmodified Rb, immunohistochemistry reveals activated cell cycle regions in tissues, and flow cytometry quantifies phosphorylation dynamics in cell populations. These applications make the antibody indispensable for cancer biology and cell cycle research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-Rb (Ser780) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Rb - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Rb - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Rb - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/Rb - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Rb+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=Rb --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047321330029,"sku":"FY13298","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_82bff8c2-35bb-4322-9d33-8cc24e556e73.jpg?v=1772019424","url":"https:\/\/www.ebiohippo.com\/products\/phospho-rb-ser780-antibody-retinoblastoma-bha17136200","provider":"BioHippo","version":"1.0","type":"link"}