{"product_id":"phospho-rip3-ser232-antibody-ripk3-bha17136248","title":"Phospho-RIP3 (Ser232) Antibody \/ RIPK3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-RIP3 (Ser232) Antibody \/ RIPK3 is a anti-RIP3 (phospho-Ser232) Rabbit antibody Recombinant Rabbit Monoclonal clone 31R36 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e RIP3 (phospho site: Ser232)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31R36, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-RIP3 (Ser232) antibody detects RIP3 phosphorylated at serine 232, encoded by the RIPK3 gene. RIP3, also called Receptor interacting serine threonine kinase 3, is a critical mediator of necroptosis, a regulated form of necrotic cell death. Phospho-RIP3 (Ser232) antibody provides researchers with a specific reagent to study necroptotic signaling, inflammation, and disease mechanisms involving programmed necrosis.\u003cbr\u003e\u003cbr\u003eRIP3 functions as a core signaling kinase within the necrosome complex. Research using Phospho-RIP3 (Ser232) antibody has shown that phosphorylation at Ser232 is essential for RIP3 activation and subsequent recruitment of MLKL, the executioner protein of necroptosis. This phosphorylation serves as a molecular switch controlling progression of the necroptotic pathway.\u003cbr\u003e\u003cbr\u003eStudies with Phospho-RIP3 (Ser232) antibody have revealed that necroptosis is triggered by tumor necrosis factor signaling, viral infection, and cellular stress conditions. RIP3 phosphorylation at Ser232 enables necrosome assembly, MLKL oligomerization, and disruption of plasma membrane integrity. This process releases intracellular contents that amplify inflammation and immune responses.\u003cbr\u003e\u003cbr\u003eIn disease contexts, dysregulated necroptosis contributes to pathology. Research using Phospho-RIP3 (Ser232) antibody has shown that excessive activation promotes tissue damage in conditions such as ischemia reperfusion injury, inflammatory bowel disease, and neurodegeneration. Conversely, insufficient necroptosis facilitates viral persistence and impaired immune clearance. These findings highlight RIP3 phosphorylation as a therapeutic target for modulating necroptotic signaling.\u003cbr\u003e\u003cbr\u003ePhospho-RIP3 (Ser232) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated RIP3 from total levels, immunohistochemistry localizes activated necroptotic signaling in tissue, and immunofluorescence reveals subcellular distribution during necrosome formation. These applications make the antibody valuable in cell death research.\u003cbr\u003e\u003cbr\u003eBy providing validated Phospho-RIP3 (Ser232) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=RIP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=RIP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=RIP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/RIP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=RIP3+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=RIP3 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322640749,"sku":"FY13346","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_90ac8b19-0349-4eef-9b96-2c545fc81f84.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/phospho-rip3-ser232-antibody-ripk3-bha17136248","provider":"BioHippo","version":"1.0","type":"link"}