{"product_id":"phospho-smad1-5-9-antibody-ps463-ps465-ps467-bha17130264","title":"Phospho-Smad1\/5\/9 Antibody (pS463\/pS465\/pS467)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003ePhospho-Smad1\/5\/9 Antibody (pS463\/pS465\/pS467) is an antibody targeting \u003cstrong\u003ePhospho-Smad1\/5\/9\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Phospho-Smad1\/5\/9.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Recombinant Rabbit Monoclonal; Clone HAF-19; Rabbit IgG.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSMADs comprise a family of structurally similar proteins that are the main signal transducers for receptors of the transforming growth factor beta (TGF-B) superfamily, which are critically important for regulating cell development and growth. [Wiki]\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt search: Phospho-Smad1\/5\/9 — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=Phospho-Smad1\/5\/9 - Gene search: Phospho-Smad1\/5\/9 — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Phospho-Smad1\/5\/9 - Ensembl search: Phospho-Smad1\/5\/9 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Phospho-Smad1\/5\/9 - PubMed search: Phospho-Smad1\/5\/9 antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Phospho-Smad1\/5\/9+antibody - Reactome search: Phospho-Smad1\/5\/9 — Reactome — https:\/\/reactome.org\/content\/query?q=Phospho-Smad1\/5\/9 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53046488498541,"sku":"RQ8710","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_449a1362-ced6-4266-9a7a-f50b39099104.jpg?v=1772000904","url":"https:\/\/www.ebiohippo.com\/products\/phospho-smad1-5-9-antibody-ps463-ps465-ps467-bha17130264","provider":"BioHippo","version":"1.0","type":"link"}