{"product_id":"phospho-sp1-thr453-antibody-bha12900644","title":"Phospho-SP1 (Thr453) Antibody","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003ePhospho-SP1 (Thr453) Antibody detects endogenous levels of SP1 only when phosphorylated at Threonine 453. This is a \u003cstrong\u003epolyclonal\u003c\/strong\u003e \u003cstrong\u003eRabbit\u003c\/strong\u003e anti-SP1 antibody, supplied as unconjugated, validated for \u003cstrong\u003eIF\/ICC | IHC | WB\u003c\/strong\u003e.\u003c\/p\u003e\n\n\u003ch2\u003eValidated Applications \u0026amp; Dilution Guide\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWB\u003c\/td\u003e\n\u003ctd\u003e1:500-1:2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIHC\u003c\/td\u003e\n\u003ctd\u003e1:50-1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIF\/ICC\u003c\/td\u003e\n\u003ctd\u003e1:100-1:500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eAntibody Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Protein\u003c\/th\u003e\n\u003ctd\u003eSP1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eAffinity Purified (Phospho-Peptide)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog No.\u003c\/th\u003e\n\u003ctd\u003eAF3121\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n\u003cp\u003eA synthesized peptide derived from human SP1 around the phosphorylation site of Thr453.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePredicted cross-reactivity:\u003c\/strong\u003e Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus. Predictions are based on sequence homology and have not been empirically validated unless stated otherwise.\u003c\/p\u003e\n\u003ch2\u003eValidation \u0026amp; Citations\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✓ Affinity Purified\u003c\/span\u003e  \u003cspan class=\"bhp-qc-badge\"\u003e✓ 1 Citation\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eRRID: \u003cstrong\u003eAB_2834556\u003c\/strong\u003e — registered in the \u003ca href=\"https:\/\/antibodyregistry.org\/AB_search?q=AB_2834556\" target=\"_blank\"\u003eAntibody Registry\u003c\/a\u003e for unambiguous reagent identification across publications.\u003c\/p\u003e\n\u003cp\u003eThis antibody has been independently cited in \u003cstrong\u003e1 peer-reviewed publication\u003c\/strong\u003e. Verify records via the Antibody Registry (AB_2834556) or PubMed.\u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStore at -20 °C. Stable for 12 months from date of receipt.\u003c\/p\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not intended for use in diagnostic or therapeutic procedures.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AFFINITY BIOSCIENCES LTD","offers":[{"title":"100 uL","offer_id":53327690400109,"sku":"AF3121-100UL","price":280.0,"currency_code":"USD","in_stock":true},{"title":"200 uL","offer_id":53327729459565,"sku":"AF3121-200UL","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/af-thumb400x400_a5d8a66f-38d0-4507-acef-ff27ba96e0b6.jpg?v=1778727120","url":"https:\/\/www.ebiohippo.com\/products\/phospho-sp1-thr453-antibody-bha12900644","provider":"BioHippo","version":"1.0","type":"link"}