{"product_id":"phospho-syk-tyr323-antibody-syk-bha17135995","title":"Phospho-Syk (Tyr323) Antibody \/ SYK","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Syk (Tyr323) Antibody \/ SYK is a anti-SYK (phospho-Tyr323) Rabbit antibody Recombinant Rabbit Monoclonal clone 32S11 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SYK (phospho site: Tyr323)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32S11, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Syk (Tyr323) antibody detects Spleen tyrosine kinase phosphorylated at tyrosine 323, encoded by the SYK gene. Spleen tyrosine kinase is a cytoplasmic non receptor tyrosine kinase that mediates signaling downstream of immunoreceptors such as the B cell receptor, Fc receptors, and integrins. Phosphorylation at tyrosine 323 is a critical modification that regulates kinase activity and adaptor interactions. Phospho-Syk (Tyr323) antibody provides a selective tool for studying immune signaling, B cell activation, and inflammatory responses.\u003cbr\u003e\u003cbr\u003eSpleen tyrosine kinase is activated when immunoreceptors are crosslinked, leading to phosphorylation of ITAM motifs and recruitment of SYK through its tandem SH2 domains. Once engaged, SYK becomes phosphorylated at multiple sites, including tyrosine 323, which enhances its catalytic activity and binding to signaling partners. Research with Phospho-Syk (Tyr323) antibody has demonstrated that this phosphorylation event promotes propagation of signaling cascades leading to calcium flux, MAP kinase activation, and transcriptional responses. These events are fundamental for lymphocyte activation and immune defense.\u003cbr\u003e\u003cbr\u003eBeyond lymphocytes, SYK plays roles in innate immune cells, including macrophages, mast cells, and neutrophils. Studies with Phospho-Syk (Tyr323) antibody have shown that phosphorylation at this site regulates Fc receptor signaling, phagocytosis, and cytokine release. Dysregulation of SYK phosphorylation has been linked to autoimmune disease, allergy, and chronic inflammation, making it a therapeutic target. Inhibitors of SYK are in clinical development for treatment of rheumatoid arthritis, lupus, and hematologic malignancies.\u003cbr\u003e\u003cbr\u003ePhosphorylation at tyrosine 323 also contributes to oncogenic signaling. Research with Phospho-Syk (Tyr323) antibody has revealed aberrant activation in leukemias and lymphomas, where it drives proliferation and survival. Monitoring phosphorylation state is therefore critical for understanding pathogenesis and evaluating targeted therapies. Because SYK functions at the intersection of immunity and cancer, its phosphorylation events remain of high biomedical importance.\u003cbr\u003e\u003cbr\u003ePhospho-Syk (Tyr323) antibody is used in western blotting, immunohistochemistry, and flow cytometry. Western blotting distinguishes phosphorylated and unphosphorylated forms, immunohistochemistry localizes activated kinase in tissue samples, and flow cytometry quantifies phosphorylation dynamics in immune cells. These approaches make Phospho-Syk (Tyr323) antibody essential for dissecting immune receptor signaling.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-Syk (Tyr323) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SYK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SYK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SYK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SYK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SYK+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=SYK --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047311663469,"sku":"FY13093","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/phospho-syk-tyr323-antibody-syk-bha17135995","provider":"BioHippo","version":"1.0","type":"link"}