{"product_id":"phospho-tuberin-ser939-antibody-tsc2-bha17135845","title":"Phospho-Tuberin (Ser939) Antibody \/ TSC2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Tuberin (Ser939) Antibody \/ TSC2 is a anti-TSC2 (phospho-Ser939) Rabbit antibody Recombinant Rabbit Monoclonal clone 32T70 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e TSC2 (phospho site: Ser939)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32T70, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Tuberin (Ser939) antibody detects the phosphorylated form of Tuberin, encoded by the TSC2 gene. Tuberin is a large cytoplasmic protein that forms a functional complex with Hamartin, encoded by TSC1, to regulate cell growth, proliferation, and survival. The TSC1 TSC2 complex acts as a GTPase activating protein for the small GTPase Rheb, thereby negatively regulating the mTORC1 pathway. Phosphorylation of Tuberin at serine 939 modulates its function and localization, making Phospho-Tuberin (Ser939) antibody a critical reagent for studying mTOR pathway regulation and its role in disease.\u003cbr\u003e\u003cbr\u003eTuberin integrates upstream signals from growth factors, nutrients, and energy status. Akt phosphorylates Tuberin at multiple residues including serine 939, leading to inhibition of the TSC1 TSC2 complex and activation of mTORC1. Research using Phospho-Tuberin (Ser939) antibody has shown that phosphorylation reduces the ability of Tuberin to suppress Rheb, resulting in increased protein synthesis and cell growth. This post translational modification thus acts as a switch between anabolic and catabolic states, linking extracellular signals to metabolic control.\u003cbr\u003e\u003cbr\u003eMutations in TSC2 cause Tuberous sclerosis complex, a genetic disorder characterized by benign tumor formation in multiple organs, epilepsy, and cognitive impairment. While disease is typically associated with truncating mutations, dysregulation of phosphorylation also impacts Tuberin activity. Studies with Phospho-Tuberin (Ser939) antibody have demonstrated that aberrant Akt signaling leads to hyperphosphorylation, promoting uncontrolled mTORC1 activation and contributing to tumorigenesis. This has made the TSC1 TSC2 pathway a target for therapies such as mTOR inhibitors, which are now widely used in clinical management of Tuberous sclerosis complex and related conditions.\u003cbr\u003e\u003cbr\u003eBeyond inherited disease, phosphorylation of Tuberin has implications in sporadic cancers, metabolic syndromes, and neurodevelopmental disorders. Overactive Akt driven phosphorylation at serine 939 contributes to tumor growth and survival in breast cancer, prostate cancer, and glioblastoma. Research using Phospho-Tuberin (Ser939) antibody allows detection of these changes, providing a biomarker for pathway activation. In metabolic disease, altered mTOR regulation contributes to insulin resistance and type 2 diabetes, further expanding the relevance of Tuberin phosphorylation in human health.\u003cbr\u003e\u003cbr\u003ePhospho-Tuberin (Ser939) antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated from non phosphorylated protein, providing a measure of pathway activity. Immunohistochemistry highlights tissue specific distribution of phosphorylated Tuberin in disease samples, while immunofluorescence visualizes subcellular localization changes after growth factor stimulation. Together, these approaches help define how Tuberin phosphorylation controls mTORC1 signaling in diverse cellular contexts.\u003cbr\u003e\u003cbr\u003eBy supplying Phospho-Tuberin (Ser939) antibody,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=TSC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=TSC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=TSC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/TSC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=TSC2+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=TSC2 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047307764077,"sku":"FY12943","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4b513466-446f-4459-a03e-1e9068936c0d.jpg?v=1782237064","url":"https:\/\/www.ebiohippo.com\/products\/phospho-tuberin-ser939-antibody-tsc2-bha17135845","provider":"BioHippo","version":"1.0","type":"link"}