{"product_id":"phospho-ulk1-ser556-antibody-unc-51-like-autophagy-activating-kinase-1-bha17136133","title":"Phospho-ULK1 (Ser556) Antibody \/ Unc-51 like autophagy activating kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-ULK1 (Ser556) Antibody \/ Unc-51 like autophagy activating kinase 1 is a anti-ULK1 (phospho-Ser556) Rabbit antibody Recombinant Rabbit Monoclonal clone 31U30 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ULK1 (phospho site: Ser556)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31U30, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IF, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-ULK1 (Ser556) antibody detects Unc-51 like autophagy activating kinase 1 phosphorylated at serine 556, encoded by the ULK1 gene. ULK1 is a serine\/threonine kinase that initiates autophagy in response to nutrient status and energy stress. Phosphorylation at Ser556 is an important regulatory event that integrates upstream signaling pathways to control autophagosome formation. Phospho-ULK1 (Ser556) antibody provides researchers with a highly specific reagent to study the regulation of autophagy and its role in physiology and disease.\u003cbr\u003e\u003cbr\u003eULK1 functions as the mammalian homolog of yeast Atg1, acting at the top of the autophagy hierarchy. Research using Phospho-ULK1 (Ser556) antibody has demonstrated that phosphorylation at serine 556 by AMP activated protein kinase promotes activation of ULK1 under conditions of energy depletion. This modification stimulates autophagy initiation by enabling the ULK1 complex to recruit downstream autophagy machinery. By contrast, mTORC1 signaling suppresses ULK1 through phosphorylation at other sites, highlighting the dynamic control of ULK1 activity by nutrient and energy signals.\u003cbr\u003e\u003cbr\u003ePhospho-ULK1 (Ser556) antibody has been used to show that phosphorylation at this site acts as a molecular switch for autophagy induction. In starvation or low energy states, phosphorylation at Ser556 ensures cells adapt by recycling cytoplasmic contents through lysosomal degradation. This process provides metabolic substrates for survival and maintains homeostasis. Inhibiting phosphorylation at Ser556 impairs autophagy, underscoring its essential role in this pathway.\u003cbr\u003e\u003cbr\u003eDysregulation of ULK1 phosphorylation has been linked to human disease. Studies with Phospho-ULK1 (Ser556) antibody have revealed that impaired autophagy contributes to neurodegenerative diseases, metabolic disorders, and cancer. In cancer, altered ULK1 phosphorylation influences tumor cell survival under stress conditions, promoting growth in nutrient-poor environments. In neurodegeneration, reduced ULK1 activation leads to accumulation of toxic proteins. These findings emphasize the clinical importance of monitoring ULK1 phosphorylation states.\u003cbr\u003e\u003cbr\u003ePhospho-ULK1 (Ser556) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals phosphorylation-dependent shifts in ULK1 activity, immunohistochemistry highlights tissue-specific patterns of autophagy activation, and immunofluorescence demonstrates dynamic localization of phosphorylated ULK1 at autophagosome initiation sites. These applications make Phospho-ULK1 (Ser556) antibody an indispensable reagent in autophagy research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Phospho-ULK1 (Ser556) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ULK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ULK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ULK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ULK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ULK1+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=ULK1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047319986541,"sku":"FY13231","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_24315e7b-3d31-4ea8-bb52-f01ba38ec6d0.jpg?v=1772019415","url":"https:\/\/www.ebiohippo.com\/products\/phospho-ulk1-ser556-antibody-unc-51-like-autophagy-activating-kinase-1-bha17136133","provider":"BioHippo","version":"1.0","type":"link"}