{"product_id":"phospho-vimentin-ser39-antibody-vim-bha17135499","title":"Phospho-Vimentin (Ser39) Antibody \/ VIM","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePhospho-Vimentin (Ser39) Antibody \/ VIM is a anti-VIM (phospho-Ser39) Rabbit antibody Recombinant Rabbit Monoclonal clone 31V35 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e VIM (phospho site: Ser39)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31V35, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePhospho-Vimentin (Ser39) antibody detects vimentin phosphorylated at serine 39. Vimentin, encoded by the VIM gene, is a type III intermediate filament protein that provides structural support and regulates cellular architecture. Vimentin filaments undergo dynamic phosphorylation at multiple sites, which regulates filament assembly, disassembly, and interactions with signaling proteins. Phosphorylation at Ser39 by kinases such as PKC alters filament organization during mitosis, migration, and stress responses.\u003cbr\u003e\u003cbr\u003ePhospho-Vimentin (Ser39) antibody is widely applied in cancer research, cell biology, and developmental studies. Vimentin phosphorylation at Ser39 facilitates cytoskeletal reorganization, promoting epithelial to mesenchymal transition, migration, and invasion. In cancer, elevated phospho vimentin correlates with metastasis and poor prognosis. By detecting phosphorylation at Ser39, researchers can investigate how cytoskeletal remodeling contributes to tumor progression and cell plasticity.\u003cbr\u003e\u003cbr\u003e Western blot assays reveal phosphorylated vimentin bands distinct from total protein. Immunohistochemistry highlights phospho vimentin in invasive tumor regions, while immunofluorescence maps cytoplasmic distribution in migrating cells. These applications make Phospho-Vimentin (Ser39) antibody valuable for tracking cytoskeletal changes in disease and development.\u003cbr\u003e\u003cbr\u003ePhosphorylation of vimentin integrates mechanical and chemical signals that regulate mitosis, adhesion, and stress adaptation. By applying Phospho-Vimentin (Ser39) antibody, scientists can evaluate how phosphorylation controls filament turnover and supports cellular responses to growth factors and mechanical stress. Its use in combination with total vimentin antibody provides a complete picture of cytoskeletal regulation.\u003cbr\u003e\u003cbr\u003eBeyond cancer, phospho vimentin is important in wound healing, embryogenesis, and inflammation. Dynamic filament remodeling ensures proper migration of fibroblasts, endothelial cells, and immune cells during tissue repair and development. The phospho-specific antibody therefore provides a versatile tool for exploring structural regulation in multiple biological systems.\u003cbr\u003e\u003cbr\u003ePhospho-Vimentin (Ser39) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eQuantifying post-translational regulation (including phosphorylation) alongside total protein levels.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003ePhospho-site readouts are condition-dependent and are often compared to total target levels when available.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=VIM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=VIM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=VIM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/VIM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=VIM+review - PhosphoSitePlus search — Cell Signaling Technology — https:\/\/www.phosphosite.org\/search?query=VIM --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047293706605,"sku":"FY12596","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_efb63d7d-05a2-44f9-96c4-4d499b4e1247.jpg?v=1772019314","url":"https:\/\/www.ebiohippo.com\/products\/phospho-vimentin-ser39-antibody-vim-bha17135499","provider":"BioHippo","version":"1.0","type":"link"}