{"product_id":"phtx-74-bhb21300264","title":"PhTx-74","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e (S)-N-[7-[(4-Aminobutyl)amino]heptyl]-4-hydroxy-α-[(1-oxobutyl)amino]benzenepropanamide dihydrochloride.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Philanthotoxin-7,4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 401601-12-5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C24H44Cl2N4O3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99% (HPLC)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 0.1-100 μM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e Water. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AMPA receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e PhTx-74 (Philanthotoxin-7,4) is a synthetic polyamine analogue of the naturally occurring wasp venom toxin philanthotoxin-4,3,3. It is a potent and selective antagonist of certain isoforms of the AMPA type of ionotropic glutamate receptors1. PhTx-74 inhibited GluR1 and GluR3 homomers as well as GluR1\/GluR2 where 100 μM inhibited nearly 100% of the glutamate evoked currents. However, up to 500 μM had no or little effect on GluR2 or GluR2\/GluR3 channels1-2.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAlternative names:\u003c\/strong\u003e Philanthotoxin-7,4\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003ePhTx-74 (Philanthotoxin-7,4) is a synthetic polyamine analogue of the naturally occurring wasp venom toxin philanthotoxin-4,3,3. It is a potent and selective antagonist of certain isoforms of the AMPA type of ionotropic glutamate receptors1.PhTx-74 inhibited GluR1 and GluR3 homomers as well as GluR1\/GluR2 where 100 µM inhibited nearly 100% of the glutamate evoked currents. However, up to 500 µM had no or little effect on GluR2 or GluR2\/GluR3 channels1-2.PhTx-74 (5 µM) was used as a tool to demonstrate circadian changes in AMPAR subtypes functionally expressed in somatosensory cortex slices and the removal of some AMPAR during sleep.Indeed, EPSPs were significantly depressed by PhTx-74 only after dark episodes3. As a measure for changes in AMPAR subunit composition after cocaine administration, PhTx-74 was used in synaptic transmission evaluation. AMPAR EPSCs from neurons treated earlier with cocaine showed increased sensitivity to bath application of 100 µM PhTx-74 compared with vehicle-treated cells, suggesting an increased contribution of GluR1-containing AMPARs to synaptic transmission after cocaine4.PhTx-74 was used to evaluate the effects of transmembrane AMPAR regulatory proteins (TARPs) on AMPAR conductance; 100 µM PhTx-74 blocked GluA2\/A4 currents to a different degree depending on the auxiliary TARP present5.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"1 mg \/ 1","offer_id":53249712095597,"sku":"P-120-1MG-1","price":55.0,"currency_code":"USD","in_stock":true},{"title":"10 mg \/ 1","offer_id":53311569887597,"sku":"P-120-10MG-1","price":129.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311569920365,"sku":"P-120-25MG-1","price":261.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311569953133,"sku":"P-120-5MG-1","price":91.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311569985901,"sku":"P-120-50MG-1","price":448.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/P-120_str-3.gif?v=1778544444","url":"https:\/\/www.ebiohippo.com\/products\/phtx-74-bhb21300264","provider":"BioHippo","version":"1.0","type":"link"}