{"product_id":"phyhd1-antibody-phytanoyl-coa-dioxygenase-domain-containing-protein-1-bha17136104","title":"PHYHD1 Antibody \/ Phytanoyl-CoA dioxygenase domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePHYHD1 Antibody \/ Phytanoyl-CoA dioxygenase domain-containing protein 1 is a anti-PHYHD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PHYHD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePHYHD1 antibody detects Phytanoyl-CoA dioxygenase domain-containing protein 1, a putative oxidative enzyme that may play roles in lipid metabolism and cellular stress responses. The UniProt recommended name is Phytanoyl-CoA dioxygenase domain-containing protein 1 (PHYHD1). This non-heme iron-dependent enzyme is thought to participate in hydroxylation or oxidative reactions linked to fatty acid metabolism and peroxisomal function.\u003cbr\u003e\u003cbr\u003eFunctionally, PHYHD1 antibody identifies a 290-amino-acid protein localized mainly in the cytoplasm and potentially associated with peroxisomes. PHYHD1 contains a conserved 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase domain that catalyzes oxidation reactions using molecular oxygen and 2-oxoglutarate as cofactors. Although its precise substrates remain under investigation, PHYHD1 may regulate lipid processing, hypoxia response, and reactive oxygen species signaling.\u003cbr\u003e\u003cbr\u003eThe PHYHD1 gene is located on chromosome 9q34.11 and is expressed in brain, liver, and kidney. Transcription of PHYHD1 is induced by cellular stress and metabolic cues, suggesting it plays a role in adaptive metabolic regulation. Comparative genomics studies indicate conservation across vertebrates, implying fundamental metabolic importance.\u003cbr\u003e\u003cbr\u003ePathologically, alterations in PHYHD1 expression have been associated with gliomas and metabolic disorders involving peroxisomal dysfunction. Reduced PHYHD1 levels may impair fatty acid oxidation and redox homeostasis, while overexpression correlates with enhanced metabolic flexibility in cancer cells. Research using PHYHD1 antibody supports studies in lipid oxidation, metabolic adaptation, and enzymatic regulation under hypoxia.\u003cbr\u003e\u003cbr\u003ePHYHD1 antibody is validated for western blotting, immunofluorescence, and ELISA to detect 2OG-dependent oxygenases. \u003cbr\u003e\u003cbr\u003eStructurally, Phytanoyl-CoA dioxygenase domain-containing protein 1 features a double-stranded beta-helix fold typical of 2OG oxygenases, with a conserved His-Asp-His metal-binding triad that coordinates Fe(II). This structure enables catalytic hydroxylation reactions essential for lipid and oxygen metabolism. This antibody facilitates analysis of PHYHD1's role in oxidative biochemistry, peroxisomal function, and metabolic stress response.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PHYHD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PHYHD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PHYHD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PHYHD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PHYHD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318217069,"sku":"FY13202","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0b3544ea-e6ee-43ec-864d-8467dbb0686b.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/phyhd1-antibody-phytanoyl-coa-dioxygenase-domain-containing-protein-1-bha17136104","provider":"BioHippo","version":"1.0","type":"link"}