{"product_id":"picalm-antibody-phosphatidylinositol-binding-clathrin-assembly-protein-bha17135584","title":"PICALM Antibody \/ Phosphatidylinositol binding clathrin assembly protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePICALM Antibody \/ Phosphatidylinositol binding clathrin assembly protein is a anti-PICALM Rabbit antibody Recombinant Rabbit Monoclonal clone 32P30 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PICALM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P30, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePICALM antibody detects phosphatidylinositol binding clathrin assembly protein, a protein encoded by the PICALM gene. PICALM is a clathrin associated protein that regulates endocytosis by linking clathrin to membranes containing phosphatidylinositol lipids. It contributes to the formation of clathrin coated vesicles, trafficking of receptors, and synaptic vesicle recycling. PICALM is widely expressed in neuronal, hematopoietic, and epithelial tissues, reflecting its central role in membrane transport and cellular communication.\u003cbr\u003e\u003cbr\u003ePICALM antibody is widely applied in neuroscience, immunology, and oncology. In neurons, PICALM participates in synaptic vesicle endocytosis, supporting neurotransmission. In hematopoietic cells, it regulates receptor mediated endocytosis and antigen presentation. Genetic variants in PICALM are strongly associated with Alzheimer disease risk, making it a focus of neurodegenerative research. By detecting PICALM, researchers can investigate how clathrin mediated endocytosis impacts diverse physiological and pathological processes.\u003cbr\u003e\u003cbr\u003e Western blotting with PICALM antibody detects protein expression in brain and immune cell lysates. Immunohistochemistry maps distribution in neuronal tissues and lymphoid organs. Immunofluorescence highlights punctate localization at clathrin coated pits and endosomes. These assays allow detailed analysis of PICALM function in vesicular trafficking.\u003cbr\u003e\u003cbr\u003ePICALM is also important in oncology. Chromosomal translocations involving PICALM, such as PICALM AF10 fusions, are associated with acute leukemias. These fusions alter transcriptional programs and contribute to leukemogenesis. Detection of PICALM with antibody based methods supports research into oncogenic mechanisms and potential diagnostic strategies. In solid tumors, altered PICALM expression affects endocytosis of growth factor receptors and signaling pathways, influencing tumor progression.\u003cbr\u003e\u003cbr\u003eIn Alzheimer disease, genome wide association studies have identified PICALM variants as risk factors. Mechanistically, PICALM regulates endocytosis of amyloid precursor protein and clearance of amyloid beta. Altered PICALM expression affects synaptic function and neuronal survival, linking endocytosis defects to neurodegeneration. By applying PICALM antibody, scientists can explore these mechanisms and evaluate therapeutic strategies targeting endocytic pathways.\u003cbr\u003e\u003cbr\u003ePICALM also contributes to immune regulation. In antigen presenting cells, it modulates receptor trafficking and signaling. Its role in membrane dynamics extends to viral infections, where viruses exploit clathrin mediated endocytosis for entry. PICALM antibody therefore provides a tool for studying both host defense and viral pathogenesis.\u003cbr\u003e\u003cbr\u003eBeyond its cellular functions, PICALM has structural features that enable its roles in trafficking. It contains phosphatidylinositol binding domains and clathrin binding motifs, which link membrane lipids to clathrin coat assembly. It also interacts with adaptor proteins and other regulators of endocytosis. These interactions highlight the versatility of PICALM as a scaffold protein coordinating vesicle formation.\u003cbr\u003e\u003cbr\u003ePICALM antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PICALM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PICALM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PICALM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PICALM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PICALM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297343853,"sku":"FY12681","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cdf5d9de-244b-49d4-849b-4342957f73d5.jpg?v=1772019329","url":"https:\/\/www.ebiohippo.com\/products\/picalm-antibody-phosphatidylinositol-binding-clathrin-assembly-protein-bha17135584","provider":"BioHippo","version":"1.0","type":"link"}