{"product_id":"pik3c3-antibody-vps34-phosphatidylinositol-3-kinase-catalytic-subunit-type-3-bha17135977","title":"PIK3C3 Antibody \/ VPS34 \/ Phosphatidylinositol 3-kinase catalytic subunit type 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePIK3C3 Antibody \/ VPS34 \/ Phosphatidylinositol 3-kinase catalytic subunit type 3 is a anti-PIK3C3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PIK3C3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePIK3C3 antibody detects Phosphatidylinositol 3-kinase catalytic subunit type 3, a lipid kinase that generates phosphatidylinositol 3-phosphate (PI3P) to control autophagy and endosomal trafficking. The UniProt recommended name is Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), also known as Vacuolar protein sorting-associated protein 34 (VPS34). This enzyme is the only class III PI3K in mammalian cells and plays a pivotal role in autophagosome formation and lysosomal function.\u003cbr\u003e\u003cbr\u003eFunctionally, PIK3C3 antibody recognizes a 887-amino-acid cytoplasmic protein that catalyzes the phosphorylation of phosphatidylinositol to generate PI3P, recruiting downstream effectors such as WIPI and DFCP1. PIK3C3 forms multiprotein complexes with Beclin 1, UVRAG, ATG14, and VPS15 to regulate membrane nucleation during autophagy and endosome maturation. Its lipid kinase activity is crucial for endocytic trafficking, nutrient sensing, and organelle biogenesis.\u003cbr\u003e\u003cbr\u003eThe PIK3C3 gene, located on chromosome 18q12.3, is ubiquitously expressed and evolutionarily conserved from yeast to humans. It governs autophagic flux by mediating vesicle formation and fusion with lysosomes. Through its interaction with Beclin 1, PIK3C3 serves as a core regulator of cellular homeostasis under nutrient stress and is involved in tumor suppression and neuroprotection.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of PIK3C3\/VPS34 contributes to cancer, neurodegeneration, and infectious disease. Inhibition of VPS34 impairs autophagy, leading to protein aggregation and metabolic imbalance. Conversely, enhanced PIK3C3 activity supports tumor cell survival under nutrient deprivation. The enzyme is also hijacked by viruses to generate replication compartments. Research using PIK3C3 antibody advances understanding of autophagic control, endosomal transport, and signaling adaptation to stress.\u003cbr\u003e\u003cbr\u003ePIK3C3 antibody is suitable for western blotting, immunofluorescence, and immunoprecipitation to detect VPS34 and its complexes. It supports studies in autophagy, intracellular trafficking, and lipid signaling. \u003cbr\u003e\u003cbr\u003eStructurally, PIK3C3 consists of a helical domain, a C2 domain for membrane association, and a lipid kinase catalytic domain. Its activity depends on interactions with VPS15 and Beclin 1 for stabilization and subcellular localization. This antibody facilitates analysis of PIK3C3\/VPS34's central role in autophagy initiation and endolysosomal trafficking.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PIK3C3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PIK3C3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PIK3C3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PIK3C3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PIK3C3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311630701,"sku":"FY13075","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fff53d7d-5f8b-46f6-b700-c4b73abdf089.jpg?v=1782237090","url":"https:\/\/www.ebiohippo.com\/products\/pik3c3-antibody-vps34-phosphatidylinositol-3-kinase-catalytic-subunit-type-3-bha17135977","provider":"BioHippo","version":"1.0","type":"link"}