{"product_id":"pip4k2b-antibody-phosphatidylinositol-5-phosphate-4-kinase-type-2-beta-bha17135493","title":"PIP4K2B Antibody \/ Phosphatidylinositol-5-phosphate 4-kinase type-2 beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePIP4K2B Antibody \/ Phosphatidylinositol-5-phosphate 4-kinase type-2 beta is a anti-PIP4K2B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PIP4K2B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePIP4K2B antibody detects Phosphatidylinositol-5-phosphate 4-kinase type-2 beta, a lipid kinase involved in the generation of phosphatidylinositol-4,5-bisphosphate (PIP2), a key signaling molecule regulating membrane trafficking, cytoskeletal organization, and nuclear signaling. PIP4K2B phosphorylates phosphatidylinositol-5-phosphate (PI5P) at the 4-position to produce PIP2, controlling both cytoplasmic and nuclear pools of phosphoinositides. The PIP4K2B antibody is used in cell signaling and lipid metabolism research to study phosphoinositide regulation and signaling compartmentalization.\u003cbr\u003e\u003cbr\u003ePIP4K2B is encoded by the PIP4K2B gene located on human chromosome 17q11.2. The protein is approximately 416 amino acids in length and belongs to the type II phosphatidylinositol 4-kinase family. PIP4K2B localizes to both the cytoplasm and nucleus, dynamically shuttling in response to growth factor stimulation and metabolic status. Its nuclear localization is critical for maintaining gene expression through phosphoinositide-dependent transcriptional regulation.\u003cbr\u003e\u003cbr\u003eThe PIP4K2B antibody detects a 47 kilodalton protein by western blot and reveals cytoplasmic and nuclear staining under confocal microscopy. PIP4K2B cooperates with its isoforms PIP4K2A and PIP4K2C to maintain phosphoinositide homeostasis. It modulates nuclear PIP2 levels, influencing chromatin remodeling, mRNA processing, and DNA damage responses. In the cytoplasm, PIP4K2B regulates endosomal trafficking and actin cytoskeleton reorganization, integrating lipid metabolism with cell motility.\u003cbr\u003e\u003cbr\u003eFunctionally, PIP4K2B connects metabolic signaling with growth control through crosstalk between phosphoinositide and mTOR pathways. Loss of PIP4K2B sensitizes cells to metabolic stress and DNA damage, while its overexpression enhances proliferation and survival in cancer cells. Mutations or reduced expression of PIP4K2B have been linked to metabolic disorders, insulin resistance, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eBecause of its dual role in lipid signaling and nuclear regulation, PIP4K2B is a key node in phosphoinositide-mediated signal integration.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PIP4K2B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PIP4K2B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PIP4K2B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PIP4K2B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PIP4K2B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294165357,"sku":"FY12590","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7f464a80-753f-4502-aef2-f5d82c2f4d70.jpg?v=1782237029","url":"https:\/\/www.ebiohippo.com\/products\/pip4k2b-antibody-phosphatidylinositol-5-phosphate-4-kinase-type-2-beta-bha17135493","provider":"BioHippo","version":"1.0","type":"link"}