{"product_id":"pip5k1a-antibody-phosphatidylinositol-4-phosphate-5-kinase-type-1-alpha-bha17135926","title":"PIP5K1A Antibody \/ Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePIP5K1A Antibody \/ Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha is a anti-PIP5K1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PIP5K1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePIP5K1A antibody detects Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, a lipid kinase responsible for generating phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a key regulator of membrane trafficking and signal transduction. The UniProt recommended name is Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha (PIP5K1A). This enzyme is part of the phosphoinositide kinase family, which coordinates membrane dynamics and cytoskeletal organization.\u003cbr\u003e\u003cbr\u003eFunctionally, PIP5K1A antibody identifies a 562-amino-acid enzyme that catalyzes the phosphorylation of phosphatidylinositol 4-phosphate at the D5 position, generating PI(4,5)P2 at the plasma membrane. This phospholipid acts as a precursor for multiple signaling molecules, including IP3, DAG, and PI(3,4,5)P3, and serves as a membrane docking site for proteins involved in vesicle trafficking, actin remodeling, and receptor signaling. PIP5K1A regulates endocytosis, exocytosis, and focal adhesion turnover through spatial control of PI(4,5)P2 production.\u003cbr\u003e\u003cbr\u003eThe PIP5K1A gene is located on chromosome 1q21.3 and encodes a ubiquitously expressed enzyme enriched in the plasma membrane and perinuclear regions. PIP5K1A functions together with its isoforms PIP5K1B and PIP5K1C to maintain distinct pools of PI(4,5)P2 for different cellular processes. It interacts with small GTPases such as ARF6 and Rac1 to coordinate signaling at the membrane-cytoskeleton interface. Dysregulation of PIP5K1A disrupts cell polarity, vesicle trafficking, and growth factor signaling, contributing to diseases such as cancer and neurodegeneration.\u003cbr\u003e\u003cbr\u003eIn cell signaling, PIP5K1A modulates receptor-mediated activation of PI3K and PLC pathways, influencing calcium flux, membrane potential, and cytoskeletal remodeling. Its role in actin polymerization makes it essential for cell migration and adhesion dynamics. PIP5K1A activity is also linked to insulin signaling, where it regulates GLUT4 vesicle trafficking and glucose uptake in adipocytes and muscle cells.\u003cbr\u003e\u003cbr\u003ePIP5K1A antibody is widely used in cell signaling, membrane biology, and lipid metabolism research. It is suitable for immunoblotting, immunofluorescence, and lipid kinase assays to analyze PIP5K1A expression and localization. This antibody aids in studying phosphoinositide regulation, cytoskeletal remodeling, and endocytic trafficking. In disease research, it supports investigations into cancer metastasis, metabolic regulation, and neuronal plasticity.\u003cbr\u003e\u003cbr\u003eStructurally, PIP5K1A contains an N-terminal kinase domain with ATP- and substrate-binding sites, regulatory motifs for localization, and autophosphorylation sites that control activity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PIP5K1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PIP5K1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PIP5K1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PIP5K1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PIP5K1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309238637,"sku":"FY13024","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_32295363-98d6-4b56-bcbe-d3bd0a621502.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/pip5k1a-antibody-phosphatidylinositol-4-phosphate-5-kinase-type-1-alpha-bha17135926","provider":"BioHippo","version":"1.0","type":"link"}