{"product_id":"pip5k1b-antibody-phosphatidylinositol-4-phosphate-5-kinase-type-1-beta-bha17135569","title":"PIP5K1B Antibody \/ Phosphatidylinositol-4-phosphate 5-kinase type 1 beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePIP5K1B Antibody \/ Phosphatidylinositol-4-phosphate 5-kinase type 1 beta is a anti-PIP5K1B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PIP5K1B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePIP5K1B antibody detects Phosphatidylinositol-4-phosphate 5-kinase type 1 beta, a lipid kinase that catalyzes the phosphorylation of phosphatidylinositol-4-phosphate to generate phosphatidylinositol-4,5-bisphosphate, a key signaling lipid controlling actin dynamics and membrane trafficking. PIP5K1B regulates cellular architecture, vesicle transport, and signal transduction by producing pools of phosphatidylinositol-4,5-bisphosphate that recruit actin-binding and signaling proteins to membranes. The PIP5K1B antibody is widely used in cell signaling, neurobiology, and cancer research to study phosphoinositide metabolism and actin regulation.\u003cbr\u003e\u003cbr\u003ePIP5K1B is encoded by the PIP5K1B gene on human chromosome 9q34.11. The protein is approximately 540 amino acids long and localizes mainly to the plasma membrane, with additional pools at the Golgi and endosomal compartments. As a member of the phosphatidylinositol-4-phosphate 5-kinase type I family, PIP5K1B works in concert with its isoforms PIP5K1A and PIP5K1C to sustain local lipid signaling domains that control cytoskeletal remodeling and vesicular trafficking.\u003cbr\u003e\u003cbr\u003eThe PIP5K1B antibody detects a 68 kilodalton band by western blot and shows strong plasma-membrane enrichment under immunofluorescence microscopy. PIP5K1B contributes to the organization of stress fibers and focal adhesions by controlling actin polymerization. It also modulates membrane ruffling, clathrin-mediated endocytosis, and vesicle docking. In neurons, PIP5K1B maintains synaptic vesicle recycling and presynaptic architecture by sustaining phosphatidylinositol-4,5-bisphosphate-dependent protein interactions.\u003cbr\u003e\u003cbr\u003eMutations or reduced expression of PIP5K1B have been associated with parkinsonism-dystonia syndromes, where impaired phosphoinositide metabolism leads to defective membrane trafficking and axonal degeneration. Dysregulation of PIP5K1B activity also contributes to cancer cell invasion through enhanced focal-adhesion turnover and migration. As a central regulator of lipid-signaling microdomains, PIP5K1B acts as a molecular hub linking mechanical and biochemical cues at the cell membrane.\u003cbr\u003e\u003cbr\u003eBecause PIP5K1B integrates phospholipid metabolism with cytoskeletal regulation, it provides insight into how membrane signaling orchestrates cellular architecture and motility.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PIP5K1B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PIP5K1B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PIP5K1B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PIP5K1B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PIP5K1B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296819565,"sku":"FY12666","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e4850868-447a-498f-b813-b65d9a063e45.jpg?v=1782237046","url":"https:\/\/www.ebiohippo.com\/products\/pip5k1b-antibody-phosphatidylinositol-4-phosphate-5-kinase-type-1-beta-bha17135569","provider":"BioHippo","version":"1.0","type":"link"}