{"product_id":"pipox-antibody-peroxisomal-sarcosine-oxidase-bha17135941","title":"PIPOX Antibody \/ Peroxisomal sarcosine oxidase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePIPOX Antibody \/ Peroxisomal sarcosine oxidase is a anti-PIPOX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Peroxisome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PIPOX\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePIPOX antibody detects Peroxisomal sarcosine oxidase, a flavin-dependent enzyme involved in the oxidative demethylation of sarcosine and pipecolate in peroxisomes. The UniProt recommended name is Peroxisomal sarcosine oxidase (PIPOX). This enzyme participates in amino acid catabolism and contributes to cellular redox homeostasis through hydrogen peroxide production during substrate oxidation.\u003cbr\u003e\u003cbr\u003eFunctionally, PIPOX antibody identifies a 438-amino-acid peroxisomal matrix enzyme that catalyzes the conversion of sarcosine to glycine, formaldehyde, and hydrogen peroxide. PIPOX contains a covalently bound FAD cofactor that mediates electron transfer to molecular oxygen during oxidative demethylation. It also oxidizes L-pipecolate, a lysine degradation intermediate, linking it to amino acid metabolism and detoxification of secondary amines.\u003cbr\u003e\u003cbr\u003eThe PIPOX gene is located on chromosome 17p13.3 and encodes a protein expressed in liver, kidney, and muscle tissues, where peroxisomal metabolism is active. PIPOX contributes to maintaining amino acid balance and redox stability by coupling substrate oxidation with reactive oxygen species generation. In peroxisomes, PIPOX works alongside catalase to neutralize hydrogen peroxide, preventing oxidative stress accumulation.\u003cbr\u003e\u003cbr\u003ePhysiologically, PIPOX supports one-carbon metabolism by producing glycine, a precursor for glutathione synthesis and methylation reactions. Its regulation is influenced by dietary amino acid intake, metabolic state, and oxidative stress levels. Reduced PIPOX activity disrupts amino acid degradation and increases susceptibility to oxidative injury. Elevated activity has been observed in metabolic adaptations to fasting and in response to peroxisome proliferator-activated receptor (PPAR) signaling.\u003cbr\u003e\u003cbr\u003ePIPOX antibody is widely used in peroxisomal biology, amino acid metabolism, and redox regulation research. It is suitable for western blotting, immunohistochemistry, and enzyme localization assays to study PIPOX expression and function. This antibody supports studies of peroxisomal oxidative pathways, redox signaling, and metabolic adaptation. In biomedical research, PIPOX serves as a marker of peroxisomal enzymatic activity and amino acid turnover.\u003cbr\u003e\u003cbr\u003eStructurally, PIPOX contains FAD-binding and substrate-binding domains typical of the flavin oxidase family. It localizes to peroxisomes via a C-terminal peroxisomal targeting signal (PTS1).\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PIPOX - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PIPOX - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PIPOX - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PIPOX - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PIPOX+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309697389,"sku":"FY13039","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_96dd4932-0921-4241-b821-59c3670568a7.jpg?v=1782237072","url":"https:\/\/www.ebiohippo.com\/products\/pipox-antibody-peroxisomal-sarcosine-oxidase-bha17135941","provider":"BioHippo","version":"1.0","type":"link"}