{"product_id":"pkc-alpha-antibody-apkc-bha17102663","title":"PKC alpha Antibody \/ aPKC","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003ePKC alpha antibody supplied as a purified reagent for WB in Rat, Primate samples. This product is a monoclonal (mouse origin) antibody (host: Mouse; isotype: Mouse IgG2a) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Monoclonal (mouse origin); host Mouse; isotype Mouse IgG2a; clone 3G11-G11-G11.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein G affinity.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Rat, Primate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A synthetic PKC alpha\/aPKC peptide was used as the immunogen for the PKC alpha antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003ePKC alpha is the intended antigen for this primary antibody. Reported biological context includes: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003ePost-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.\u003c\/li\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P17252) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P17252\/entry - NCBI Gene search (PKC alpha) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC+alpha - Ensembl search (PKC alpha) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC+alpha - PubMed search (PKC alpha) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+alpha - Reactome pathway search (PKC alpha) — Reactome — https:\/\/reactome.org\/content\/query?q=PKC+alpha --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In PBS with 50% glycerol and 0.03% ProClin 300 \/ 0.1 ml","offer_id":53043212845421,"sku":"F54061-0.1ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_02b79804-4b22-4aa2-9b13-2e38046fd753.jpg?v=1771934136","url":"https:\/\/www.ebiohippo.com\/products\/pkc-alpha-antibody-apkc-bha17102663","provider":"BioHippo","version":"1.0","type":"link"}