{"product_id":"pkc-delta-theta-antibody-prkcd-prkcq-bha17135706","title":"PKC delta\/theta Antibody \/ PRKCD\/PRKCQ","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePKC delta\/theta Antibody \/ PRKCD\/PRKCQ is a anti-PKC Rabbit antibody Recombinant Rabbit Monoclonal clone 31P77 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKC\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31P77, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePKC delta\/theta antibody detects protein kinase C delta and protein kinase C theta, encoded by the PRKCD and PRKCQ genes. PKC delta is also called nPKC delta, while PKC theta is known as nPKC theta. Both belong to the novel PKC subfamily, which are activated by diacylglycerol but not calcium. PKC delta is broadly expressed and regulates apoptosis, proliferation, and differentiation, while PKC theta is highly enriched in T cells and is critical for T cell receptor signaling and immune synapse formation. Detecting both isoforms with a single antibody provides a versatile approach for studying PKC signaling across systems.\u003cbr\u003e\u003cbr\u003ePKC delta\/theta antibody is widely applied in immunology, cancer biology, and signal transduction studies. PKC delta acts as a tumor suppressor or promoter depending on context, influencing apoptosis, DNA damage responses, and cell cycle regulation. PKC theta is essential for T cell activation, NF kappa B signaling, and cytokine production. By detecting both isoforms, researchers can evaluate overlapping and distinct functions in immunity, cancer, and tissue homeostasis.\u003cbr\u003e\u003cbr\u003eApplications for PKC delta\/theta antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect PKC isoforms in immune and cancer cell lysates, immunohistochemistry maps expression in tissues, and immunofluorescence highlights subcellular localization at membranes and cytoskeleton. These methods support detailed characterization of PKC signaling dynamics.\u003cbr\u003e\u003cbr\u003ePKC delta contributes to apoptosis in response to DNA damage and oxidative stress, while PKC theta mediates survival signals in T cells. Dysregulated activity of either isoform contributes to autoimmunity, cancer progression, and cardiovascular disease. By applying PKC delta\/theta antibody, scientists can examine how combined PKC signaling influences disease outcomes and therapeutic responses.\u003cbr\u003e\u003cbr\u003ePKC isoforms are therapeutic targets, with inhibitors and modulators under development for cancer and immune disorders. Monitoring PKC delta and PKC theta expression provides biomarkers for pathway activity. PKC delta\/theta antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKC - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKC - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKC - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKC - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKC+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047301898605,"sku":"FY12804","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1483da27-e1ca-404b-8fa5-a2f38d4c8f7e.jpg?v=1772019347","url":"https:\/\/www.ebiohippo.com\/products\/pkc-delta-theta-antibody-prkcd-prkcq-bha17135706","provider":"BioHippo","version":"1.0","type":"link"}