{"product_id":"pkn3-antibody-protein-kinase-n3-bha17135762","title":"PKN3 Antibody \/ Protein kinase N3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePKN3 Antibody \/ Protein kinase N3 is a anti-PKN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PKN3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePKN3 antibody detects Protein kinase N3, a serine\/threonine kinase involved in cytoskeletal organization, cell adhesion, and tumorigenesis. Encoded by the PKN3 gene on chromosome 9q31.2, this enzyme is a member of the protein kinase N (PKN) family, which are effectors of the Rho family of small GTPases. PKN3 links Rho signaling to downstream pathways that regulate actin dynamics, cell migration, and proliferation, making it a key mediator of cytoskeletal remodeling and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eStructurally, PKN3 contains an N-terminal regulatory region with HR1 (homology region 1) motifs that bind Rho GTPases, followed by a C-terminal catalytic domain similar to protein kinase C (PKC). PKN3 phosphorylates substrates involved in actin filament stabilization and cell junction assembly, coordinating mechanical and adhesive cues. It is localized to the cytoplasm and plasma membrane, where it interacts with focal adhesion proteins and scaffolds to transmit signals influencing motility and growth.\u003cbr\u003e\u003cbr\u003eThe PKN3 antibody is widely used in cancer biology, cell signaling, and cytoskeletal research to investigate Rho-dependent kinase pathways and cell morphology control. Western blot analysis detects a 110 kilodalton band corresponding to PKN3, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining in epithelial and tumor cells. This antibody supports research into the molecular basis of migration, invasion, and metastatic progression.\u003cbr\u003e\u003cbr\u003ePKN3 is overexpressed in several cancers, including breast, prostate, and pancreatic carcinomas, where it promotes invasive behavior through activation of the PI3K\/AKT and RhoA pathways. Genetic or pharmacologic inhibition of PKN3 suppresses tumor growth and metastasis, making it a promising therapeutic target. Beyond oncology, PKN3 also contributes to endothelial cell barrier function and angiogenesis. The PKN3 antibody provides a valuable reagent for dissecting the cellular functions and therapeutic potential of this kinase.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PKN3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PKN3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PKN3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PKN3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PKN3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304651117,"sku":"FY12860","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_df58b257-332f-4dc8-beda-2b335179af0d.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/pkn3-antibody-protein-kinase-n3-bha17135762","provider":"BioHippo","version":"1.0","type":"link"}