{"product_id":"plaat4-antibody-rarres3-bha17135779","title":"PLAAT4 Antibody \/ RARRES3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePLAAT4 Antibody \/ RARRES3 is a anti-PLAAT4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLAAT4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePLAAT4 antibody detects Phospholipase A and acyltransferase 4, also known as Retinoic acid receptor responder protein 3 (RARRES3), a multifunctional enzyme involved in lipid metabolism, immune regulation, and tumor suppression. Encoded by the PLAAT4 gene on chromosome 11q23.3, this protein belongs to the phospholipase A\/acyltransferase family and is induced by retinoic acid signaling. PLAAT4 hydrolyzes membrane phospholipids and transfers acyl chains to form lysophospholipids and neutral lipids, influencing lipid signaling and membrane remodeling.\u003cbr\u003e\u003cbr\u003eStructurally, PLAAT4 is an approximately 18 kilodalton enzyme localized to the endoplasmic reticulum and Golgi membranes. It contains a conserved catalytic dyad characteristic of the NlpC\/P60 thiol protease superfamily, which mediates both phospholipase A and acyltransferase activities. Through its dual enzymatic function, PLAAT4 regulates cellular lipid composition, affecting membrane curvature, vesicle trafficking, and immune response modulation. Its expression is upregulated by retinoic acid and interferons, suggesting a role in antiviral defense and differentiation control.\u003cbr\u003e\u003cbr\u003eThe PLAAT4 antibody is widely used in cancer biology, immunology, and lipid signaling research to study retinoid-responsive pathways and lipid remodeling. Western blot analysis identifies a band at approximately 18 kilodaltons corresponding to PLAAT4, while immunofluorescence reveals perinuclear and vesicular localization consistent with endoplasmic reticulum distribution. This antibody enables investigation of how lipid hydrolysis and acylation influence inflammatory signaling and cellular differentiation.\u003cbr\u003e\u003cbr\u003eFunctionally, PLAAT4 acts as a tumor suppressor by antagonizing the Wnt\/beta-catenin pathway, suppressing proliferation, and promoting differentiation in epithelial tissues. It also participates in the interferon-mediated antiviral response, where its enzymatic products help modulate viral replication and immune activation. In lipid metabolism, PLAAT4 contributes to the synthesis of signaling lipids that regulate inflammation and apoptosis. Dysregulation or loss of PLAAT4 expression has been observed in cancers such as breast and colorectal carcinoma, linking it to impaired retinoid signaling and altered lipid homeostasis. The PLAAT4 antibody provides a reliable reagent for exploring these cellular processes and identifying therapeutic targets in retinoid and immune pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLAAT4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLAAT4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLAAT4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLAAT4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLAAT4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305077101,"sku":"FY12877","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_33a5befe-0497-48f7-adcd-948144581023.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/plaat4-antibody-rarres3-bha17135779","provider":"BioHippo","version":"1.0","type":"link"}