{"product_id":"plant-total-rna-extraction-kit-bht15200028","title":"Plant Total RNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe ground plant tissue is dissolved by the strong lysis solution and filtered to remove protein impurities and cell debris. Column purification technology efficiently removes residual protein and PCR inhibitors. After RNA is washed with solution RRPB and Solution RWB, it is eluted with RNase-Free ddH₂O, which can be used in various molecular biology experiments.\u003c\/p\u003e\n\u003cp\u003eA 100 mg sample is ground and lysed in 600 µL of Lysis buffer C, clarified at 12,000 rpm for 5 min, mixed with 0.3 volumes of isopropanol and loaded onto the R adsorption column at 10,000 rpm for 1 min. The column is washed with 500 µL of Solution RRPB, treated on-column with 80 µL of DNase I working solution for 10 min at room temperature, washed again with RRPB and twice with 500 µL of Wash Buffer RWB, dried at 12,000 rpm for 2 min and eluted with 30–100 µL of RNase-Free ddH₂O. The expected purity is OD260\/OD280 1.8–2.1, with 2.0 indicating high-quality RNA.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (adsorption column R)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003ePlant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e100 mg standard; 150 mg for fruits, tubers and petals; 60–100 mg for dried seeds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003eNo phenol\/chloroform extraction, cesium chloride gradient or lithium chloride\/ethanol precipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eOn-column DNase I; DNase I stock solution and DNase I Buffer RDB supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003e1.8–2.1; 2.0 indicates high-quality RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30–100 µL RNase-Free ddH₂O\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysis buffer C\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003ctd\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNA deproteinizing Solution RRPB\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I stock solution\u003c\/td\u003e\n\u003ctd\u003e525 µL\u003c\/td\u003e\n\u003ctd\u003e2.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I Buffer RDB\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer RWB\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption Column R\u003c\/td\u003e\n\u003ctd\u003e50 sets\u003c\/td\u003e\n\u003ctd\u003e200 sets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free ddH₂O\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003ctd\u003e160 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal RNA extraction from plant tissue\u003c\/li\u003e\n\u003cli\u003eRNA extraction from fruits, tubers, petals and dried seeds\u003c\/li\u003e\n\u003cli\u003eRemoval of DNA contamination with on-column DNase I\u003c\/li\u003e\n\u003cli\u003eDownstream molecular biology experiments requiring high-purity RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFormulated specifically for plant material, so the workflow is simpler than a general-purpose RNA protocol.\u003c\/li\u003e\n\u003cli\u003eEquipped with DNase I to remove DNA contamination.\u003c\/li\u003e\n\u003cli\u003eRNA has higher purity and no impurity residues, which is especially suitable for downstream experiments that require high purity.\u003c\/li\u003e\n\u003cli\u003eThe operation is safe and reliable, no phenol\/chloroform extraction, no cesium chloride gradient centrifugation, no lithium chloride or ethanol precipitation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eMost components are stored at room temperature for 1 year; the DNase I stock solution is stored at -20°C for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdd absolute ethanol to Wash Buffer RWB before use.\u003c\/li\u003e\n\u003cli\u003eIf the lysis buffer precipitates, dissolve it at 60–65°C before use.\u003c\/li\u003e\n\u003cli\u003eDo not shake vigorously when mixing with isopropanol.\u003c\/li\u003e\n\u003cli\u003eDry the column thoroughly; residual rinse solution interferes with downstream reactions.\u003c\/li\u003e\n\u003cli\u003eStore the RNA solution at -70°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP018\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200028\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512900973,"sku":"EP018-50T","price":208.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965517160813,"sku":"EP018-200T","price":390.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965517193581,"sku":"EP018-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965517226349,"sku":"EP018-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP018-a-1.jpg?v=1789419187","url":"https:\/\/www.ebiohippo.com\/products\/plant-total-rna-extraction-kit-bht15200028","provider":"BioHippo","version":"1.0","type":"link"}