{"product_id":"plcb1-antibody-phospholipase-c-beta-1-bha17135366","title":"PLCB1 Antibody \/ Phospholipase C beta 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePLCB1 Antibody \/ Phospholipase C beta 1 is a anti-PLCB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLCB1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePLCB1 antibody recognizes Phospholipase C beta 1, an enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the key second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). This reaction triggers intracellular calcium release and activates protein kinase C, integrating multiple receptor-mediated signaling cascades. Phospholipase C beta 1 functions downstream of G-protein-coupled receptors and plays an essential role in neuronal excitability, synaptic plasticity, and cellular growth regulation. The PLCB1 antibody is widely used to study intracellular signaling pathways, phosphoinositide metabolism, and neurological disorders involving aberrant calcium signaling.\u003cbr\u003e\u003cbr\u003ePLCB1 is encoded by the PLCB1 gene on human chromosome 20p12.3 and belongs to the phospholipase C family, which includes beta, gamma, delta, epsilon, eta, and zeta isoforms. The protein contains characteristic catalytic X and Y domains, a C2 domain responsible for calcium-dependent membrane association, and a C-terminal G-protein-binding domain. Activation occurs via interaction with Galphaq or Gbetagamma subunits of heterotrimeric G proteins. Within neurons, PLCB1 is highly expressed in the cerebral cortex, hippocampus, and striatum, where it contributes to muscarinic acetylcholine and glutamate receptor signaling. Dysregulation of PLCB1 signaling has been linked to epilepsy, schizophrenia, and bipolar disorder, emphasizing its importance in brain function.\u003cbr\u003e\u003cbr\u003eUsing the PLCB1 antibody, researchers can detect multiple isoforms of phospholipase C beta 1 ranging from 120-150 kDa depending on tissue and post-translational modifications. Western blot and immunohistochemical studies demonstrate strong neuronal cytoplasmic staining, often enriched near the plasma membrane. Functional analyses reveal that PLCB1 interacts with scaffolding proteins and cytoskeletal elements, coupling receptor activation to localized calcium dynamics and neurotransmitter release. In addition to neuronal tissues, PLCB1 is expressed in cardiac, hepatic, and endothelial cells, where it mediates G-protein-linked responses controlling vascular tone, secretion, and metabolism.\u003cbr\u003e\u003cbr\u003eLoss of PLCB1 activity impairs signal transduction, while overactivation can lead to excitotoxicity and dysregulated calcium homeostasis. Knockout models show severe neurodevelopmental defects and altered behavior, confirming its critical physiological role. Beyond the nervous system, PLCB1 contributes to immune cell activation, insulin signaling, and smooth muscle contraction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLCB1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLCB1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLCB1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLCB1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLCB1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290757485,"sku":"FY12463","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b96d7064-7a2f-4310-aae6-cd2d4a41d4f9.jpg?v=1782237016","url":"https:\/\/www.ebiohippo.com\/products\/plcb1-antibody-phospholipase-c-beta-1-bha17135366","provider":"BioHippo","version":"1.0","type":"link"}