{"product_id":"plcb2-antibody-phospholipase-c-beta-2-bha17135337","title":"PLCB2 Antibody \/ Phospholipase C-beta-2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePLCB2 Antibody \/ Phospholipase C-beta-2 is a anti-PLCB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLCB2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe PLCB2 antibody targets 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (Phospholipase C-beta-2), an enzyme encoded by the PLCB2 gene. This beta isoform of phospholipase C hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). These products regulate calcium signaling and protein kinase C activation, forming a critical pathway downstream of G-protein-coupled receptors (GPCRs) and immune receptors. The PLCB2 antibody provides a specific tool for exploring phosphoinositide signaling, calcium homeostasis, and receptor-mediated cellular activation. \u003cbr\u003e\u003cbr\u003e1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 is widely expressed in hematopoietic and epithelial cells. It interacts with G alpha q and G beta gamma subunits of heterotrimeric G-proteins, linking receptor activation to intracellular calcium mobilization. The PLCB2 antibody enables detection of this enzyme in tissues where rapid signal transduction is required, such as immune cells and neurons. Its enzymatic activity is central to processes like secretion, chemotaxis, and proliferation. \u003cbr\u003e\u003cbr\u003ePLCB2 plays a pivotal role in innate and adaptive immunity by coupling receptor engagement to downstream effector responses. The PLCB2 antibody supports investigations into Fc receptor signaling, platelet activation, and leukocyte migration. Dysregulation of phospholipase C beta-2 can lead to aberrant immune signaling, inflammation, and altered calcium dynamics that contribute to autoimmune or cardiovascular disorders. \u003cbr\u003e\u003cbr\u003eIn addition to its immune functions, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 contributes to sensory transduction in olfactory and gustatory pathways. The PLCB2 antibody is therefore valuable for neuroscience and sensory-biology research. Knockout models reveal its necessity in taste signaling and mucosal immune defense. \u003cbr\u003e\u003cbr\u003eThe PLCB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, typically revealing cytoplasmic and membrane-associated staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLCB2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLCB2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLCB2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLCB2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLCB2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288496493,"sku":"FY12434","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7b88cc4e-779a-43f3-9af7-c3f775662b29.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/plcb2-antibody-phospholipase-c-beta-2-bha17135337","provider":"BioHippo","version":"1.0","type":"link"}