{"product_id":"plcd1-antibody-phospholipase-c-delta-1-bha17136044","title":"PLCD1 Antibody \/ Phospholipase C delta 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePLCD1 Antibody \/ Phospholipase C delta 1 is a anti-PLCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLCD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePLCD1 antibody detects Phospholipase C delta 1, a signaling enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into secondary messengers inositol trisphosphate (IP3) and diacylglycerol (DAG). The UniProt recommended name is Phospholipase C delta 1 (PLCD1). This enzyme participates in calcium signaling and regulates diverse cellular processes such as growth, differentiation, and cytoskeletal reorganization.\u003cbr\u003e\u003cbr\u003eFunctionally, PLCD1 antibody identifies a 756-amino-acid enzyme containing an N-terminal PH domain for membrane binding, EF-hand motifs for calcium interaction, a catalytic X-Y domain for phosphodiesterase activity, and a C2 domain for phospholipid association. Upon receptor stimulation, PLCD1 catalyzes the cleavage of PIP2, generating IP3 to release intracellular calcium and DAG to activate protein kinase C (PKC).\u003cbr\u003e\u003cbr\u003eThe PLCD1 gene is located on chromosome 3p22.3 and is expressed in epithelial tissues, brain, and smooth muscle. It functions downstream of receptor tyrosine kinases and G protein-coupled receptors to mediate signal transduction. PLCD1 contributes to actin cytoskeleton remodeling and cell migration through localized phosphoinositide metabolism.\u003cbr\u003e\u003cbr\u003ePathologically, downregulation or mutation of PLCD1 is associated with cancer progression, skin disorders, and developmental defects. PLCD1 acts as a tumor suppressor in certain epithelial cancers by modulating calcium signaling and cell adhesion. Research using PLCD1 antibody supports studies in signal transduction, lipid metabolism, and cell communication.\u003cbr\u003e\u003cbr\u003ePLCD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect phospholipase enzymes in signaling pathways. \u003cbr\u003e\u003cbr\u003eStructurally, Phospholipase C delta 1 contains catalytic and regulatory domains that coordinate substrate recognition and calcium-dependent activity. This antibody facilitates investigation of PLCD1's role in intracellular signaling and calcium homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLCD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLCD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLCD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLCD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLCD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313531245,"sku":"FY13142","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8d2135ac-4408-4662-b11e-fe82d1d4cf2a.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/plcd1-antibody-phospholipase-c-delta-1-bha17136044","provider":"BioHippo","version":"1.0","type":"link"}