{"product_id":"pleiotrophin-antibody-ptn-bha17135326","title":"Pleiotrophin Antibody \/ PTN","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePleiotrophin Antibody \/ PTN is a anti-PTN Rabbit antibody Recombinant Rabbit Monoclonal clone 32P54 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PTN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32P54, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePleiotrophin antibody detects pleiotrophin, a secreted heparin binding growth factor encoded by the PTN gene. Pleiotrophin is a cytokine involved in the regulation of cell growth, survival, differentiation, and migration. It is highly expressed during embryonic development, where it contributes to neurogenesis, angiogenesis, and skeletal formation. In adult tissues, pleiotrophin expression is limited but becomes re induced during tissue repair and tumorigenesis.\u003cbr\u003e\u003cbr\u003ePleiotrophin antibody is applied in developmental biology, oncology, and regenerative medicine research. The protein interacts with receptors such as receptor protein tyrosine phosphatase beta zeta, syndecans, and anaplastic lymphoma kinase, activating signaling cascades including PI3K AKT and MAPK. These pathways promote proliferation and survival, making pleiotrophin an important regulator of both physiological and pathological growth. Detecting pleiotrophin provides insight into how extracellular cues influence signaling and cell fate decisions.\u003cbr\u003e\u003cbr\u003eThe antibody is validated for western blotting, immunohistochemistry, immunofluorescence, and ELISA. In western blot assays, pleiotrophin antibody identifies bands corresponding to the growth factor across tissue extracts. Immunohistochemistry reveals expression in vasculature and developing tissues, while immunofluorescence highlights extracellular distribution and receptor colocalization. ELISA applications allow quantification of secreted pleiotrophin in culture media or patient samples.\u003cbr\u003e\u003cbr\u003eOverexpression of pleiotrophin has been reported in cancers including glioblastoma, breast carcinoma, and prostate cancer. In these contexts, pleiotrophin enhances angiogenesis, invasion, and tumor progression. By applying pleiotrophin antibody, scientists can evaluate pleiotrophin levels as a biomarker and investigate its potential as a therapeutic target.\u003cbr\u003e\u003cbr\u003eIn neuroscience, pleiotrophin plays roles in neuronal survival, synaptic plasticity, and axonal regeneration after injury. It promotes neurite outgrowth and supports recovery in models of neural damage. In skeletal biology, pleiotrophin regulates osteoblast differentiation and bone growth, linking it to both normal development and fracture healing. These diverse functions demonstrate the broad research value of pleiotrophin antibody.\u003cbr\u003e\u003cbr\u003ePleiotrophin antibody provided by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PTN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PTN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PTN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PTN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PTN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047289250157,"sku":"FY12423","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b01f88d3-1a10-4432-946c-c3f731578b04.jpg?v=1772019291","url":"https:\/\/www.ebiohippo.com\/products\/pleiotrophin-antibody-ptn-bha17135326","provider":"BioHippo","version":"1.0","type":"link"}