{"product_id":"pls1-antibody-plastin-1-bha17135827","title":"PLS1 Antibody \/ Plastin-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePLS1 Antibody \/ Plastin-1 is a anti-PLS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PLS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePLS1 antibody detects Plastin-1, an actin-bundling protein that stabilizes and crosslinks actin filaments in epithelial and sensory cells. Encoded by the PLS1 gene on chromosome 3q23, this protein belongs to the plastin family of actin-bundling proteins, which includes isoforms expressed in hematopoietic, ubiquitous, and epithelial tissues. Plastin-1 plays an essential structural role in maintaining brush border microvilli in the intestinal epithelium and stereocilia in the inner ear, contributing to cellular rigidity and signal transduction.\u003cbr\u003e\u003cbr\u003eStructurally, Plastin-1 is a 630-amino-acid cytoskeletal protein of approximately 68 kilodaltons containing two tandem calponin homology (CH) domains responsible for actin binding and two EF-hand calcium-binding motifs that regulate its activity. The protein localizes predominantly to actin-rich structures such as microvilli and adherens junctions, where it bundles filamentous actin into parallel arrays that reinforce cell shape and polarity.\u003cbr\u003e\u003cbr\u003eThe PLS1 antibody is widely used in cell biology, auditory physiology, and epithelial research to study cytoskeletal architecture, microvillar dynamics, and actin filament regulation. Western blot analysis detects a 68 kilodalton band corresponding to Plastin-1, while immunofluorescence demonstrates strong staining along apical membranes in epithelial cells. This antibody provides a reliable tool for investigating actin organization and microvillar maintenance.\u003cbr\u003e\u003cbr\u003eFunctionally, Plastin-1 is critical for maintaining the structural stability of brush border and sensory epithelia by linking actin filaments into tightly packed bundles. Mutations in PLS1 cause autosomal recessive nonsyndromic hearing loss due to disruption of stereocilia structure and function in cochlear hair cells. In intestinal cells, PLS1 supports nutrient absorption and epithelial resilience under mechanical stress. The PLS1 antibody supports studies of cytoskeletal integrity, epithelial morphogenesis, and hereditary hearing loss.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PLS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PLS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PLS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PLS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PLS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306289517,"sku":"FY12925","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1ddc4bf5-a3f4-4fef-80b1-e79c275ebb71.jpg?v=1782237057","url":"https:\/\/www.ebiohippo.com\/products\/pls1-antibody-plastin-1-bha17135827","provider":"BioHippo","version":"1.0","type":"link"}